Difference between revisions of "Part:BBa K5301019"
 
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This pathway expresses MSP1E3D1 (BBa_K5301000) using T7 expression system.
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This pathway expresses MSP1E3D1 using T7 expression system.
 
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<h1>Description</h1>
 
<h1>Description</h1>
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We used this pathway to express MSP1E3D1 by using T7 expression system in <i>E.coli</i>, and the expression of MSP1E3D1 can be induced by IPTG. For more information on MSP1E3D1, go to <partinfo>BBa_K5301000</partinfo>.
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===Sequence and Features===
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K5301019 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K5301019 SequenceAndFeatures</partinfo>
  

Latest revision as of 09:32, 1 October 2024


MSP1E3D1 expression pathway (T7)
This pathway expresses MSP1E3D1 using T7 expression system.

Description

We used this pathway to express MSP1E3D1 by using T7 expression system in E.coli, and the expression of MSP1E3D1 can be induced by IPTG. For more information on MSP1E3D1, go to BBa_K5301000.

Sequence and Features



Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 173
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 173
    Illegal NheI site found at 903
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 173
    Illegal BglII site found at 799
    Illegal XhoI site found at 695
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 173
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 173
  • 1000
    COMPATIBLE WITH RFC[1000]