Difference between revisions of "Part:BBa K5302012:Design"

 
 
Line 14: Line 14:
 
===Source===
 
===Source===
  
−
This work is derived from pBBR1MCS-2, pUC19-INP and pET-21b(+)
+
INP:Pseudomonas syringae
 +
mCherry:Mushroom coral
  
 
===References===
 
===References===
 +
[1]Sonia Nicchi1,2, Maria Giuliani1 , Fabiola Giusti1 , Laura Pancotto1 , Domenico Maione1 , Isabel Delany1 ,
 +
Cesira L. Galeotti1 and Cecilia Brettoni1*.Decorating the surface of Escherichia coli with bacterial lipoproteins: a comparative analysis of diferent display systems.2021

Latest revision as of 13:26, 1 October 2024


pBBR-INP-mCherry


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 4229
    Illegal EcoRI site found at 4277
    Illegal EcoRI site found at 4550
    Illegal EcoRI site found at 4917
    Illegal XbaI site found at 3189
    Illegal PstI site found at 2016
    Illegal PstI site found at 3177
    Illegal PstI site found at 4538
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4229
    Illegal EcoRI site found at 4277
    Illegal EcoRI site found at 4550
    Illegal EcoRI site found at 4917
    Illegal PstI site found at 2016
    Illegal PstI site found at 3177
    Illegal PstI site found at 4538
    Illegal NotI site found at 1057
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4229
    Illegal EcoRI site found at 4277
    Illegal EcoRI site found at 4550
    Illegal EcoRI site found at 4917
    Illegal BglII site found at 1803
    Illegal BamHI site found at 3195
    Illegal XhoI site found at 4895
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 4229
    Illegal EcoRI site found at 4277
    Illegal EcoRI site found at 4550
    Illegal EcoRI site found at 4917
    Illegal XbaI site found at 3189
    Illegal PstI site found at 2016
    Illegal PstI site found at 3177
    Illegal PstI site found at 4538
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 4229
    Illegal EcoRI site found at 4277
    Illegal EcoRI site found at 4550
    Illegal EcoRI site found at 4917
    Illegal XbaI site found at 3189
    Illegal PstI site found at 2016
    Illegal PstI site found at 3177
    Illegal PstI site found at 4538
    Illegal NgoMIV site found at 2467
    Illegal NgoMIV site found at 2750
    Illegal NgoMIV site found at 3616
    Illegal NgoMIV site found at 5582
    Illegal AgeI site found at 4756
    Illegal AgeI site found at 5422
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1379
    Illegal SapI.rc site found at 2316
    Illegal SapI.rc site found at 2526


Design Notes

Please be advised that all listed genes have undergone codon optimization for our chassis strain, Escherichia coli Nissle 1917 (EcN). For those considering alternative chassis organisms, it is recommended to reassess the necessity of codon optimization.



Source

INP:Pseudomonas syringae mCherry:Mushroom coral

References

[1]Sonia Nicchi1,2, Maria Giuliani1 , Fabiola Giusti1 , Laura Pancotto1 , Domenico Maione1 , Isabel Delany1 , Cesira L. Galeotti1 and Cecilia Brettoni1*.Decorating the surface of Escherichia coli with bacterial lipoproteins: a comparative analysis of diferent display systems.2021