Difference between revisions of "Part:BBa K215210"
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| − | ''Cytometry showing no difference in bound biotin-conjugated fluorophore for 1mM IPTG induced vs uninduced cells at different fluorophore concentrations. Conclusion: Wild-type monomeric streptavidin shows no measureable activity when expressed as a fusion protein with | + | ''Cytometry showing no difference in bound biotin-conjugated fluorophore for 1mM IPTG induced vs uninduced cells at different fluorophore concentrations. Conclusion: Wild-type monomeric streptavidin shows no measureable activity when expressed as a fusion protein with one trans-membrane OmpA.'' |
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Latest revision as of 04:10, 22 October 2009
IPTG Inducible 1tmr OmpA Surface Display of WT Streptavidin
This part should display WT streptavidin + 6His, BBa_J36841, on the surface of the cell via the OmpA display system when induced with IPTG.
Note: in order to express this construct as a fusion protein, there is no T after this sequence and before the SpeI site (sequencing will show inconsistent).
This part doesn't show measurable activity. See [http://2009.igem.org/Team:Washington/Project/CDS#Custom_Display_Vector_with_wild-type_monomeric_streptavidin_inserted 2009 UW iGEM page] for more information.
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Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 337
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 379
Illegal AgeI site found at 430 - 1000COMPATIBLE WITH RFC[1000]