Difference between revisions of "Part:BBa K215210"
 
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This part should display WT streptavidin + 6His, BBa_J36841, on the surface of the cell via the OmpA display system when induced with IPTG.
 
This part should display WT streptavidin + 6His, BBa_J36841, on the surface of the cell via the OmpA display system when induced with IPTG.
  
−
Note: in order to express this construct as a fusion protein, there is no T after this sequence and before the SpeI site (sequencing will show inconsistant - arg!).
+
Note: in order to express this construct as a fusion protein, there is no T after this sequence and before the SpeI site (sequencing will show inconsistent).
  
 
This part doesn't show measurable activity.  See [http://2009.igem.org/Team:Washington/Project/CDS#Custom_Display_Vector_with_wild-type_monomeric_streptavidin_inserted 2009 UW iGEM page] for more information.
 
This part doesn't show measurable activity.  See [http://2009.igem.org/Team:Washington/Project/CDS#Custom_Display_Vector_with_wild-type_monomeric_streptavidin_inserted 2009 UW iGEM page] for more information.
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<table><tr align="center"><td>
 
<table><tr align="center"><td>
 
[[Image:New_generic_51.png|375px]]<br>
 
[[Image:New_generic_51.png|375px]]<br>
−
''Cytometry showing no difference in bound biotin-conjugated fluorophore for 1mM IPTG induced vs uninduced cells at different fluorophore concentrations.  Conclusion: Wild-type monomeric streptavidin shows no measureable activity when expressed as a fusion protein with 1 or 5 trans-membrane OmpA.''
+
''Cytometry showing no difference in bound biotin-conjugated fluorophore for 1mM IPTG induced vs uninduced cells at different fluorophore concentrations.  Conclusion: Wild-type monomeric streptavidin shows no measureable activity when expressed as a fusion protein with one trans-membrane OmpA.''
 
</td></tr></table>
 
</td></tr></table>
  

Latest revision as of 04:10, 22 October 2009

IPTG Inducible 1tmr OmpA Surface Display of WT Streptavidin

This part should display WT streptavidin + 6His, BBa_J36841, on the surface of the cell via the OmpA display system when induced with IPTG.

Note: in order to express this construct as a fusion protein, there is no T after this sequence and before the SpeI site (sequencing will show inconsistent).

This part doesn't show measurable activity. See [http://2009.igem.org/Team:Washington/Project/CDS#Custom_Display_Vector_with_wild-type_monomeric_streptavidin_inserted 2009 UW iGEM page] for more information.

New generic 51.png
Cytometry showing no difference in bound biotin-conjugated fluorophore for 1mM IPTG induced vs uninduced cells at different fluorophore concentrations. Conclusion: Wild-type monomeric streptavidin shows no measureable activity when expressed as a fusion protein with one trans-membrane OmpA.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 337
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 379
    Illegal AgeI site found at 430
  • 1000
    COMPATIBLE WITH RFC[1000]