Difference between revisions of "Part:BBa K5097011"
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This composite part functions as an acid sensing, base producing part. It contains the coding sequences speA and speB, the two enzymes involved in the conversion of arginine to putrescine (Morris., et al 1969). These genes, when expressed, will produce putrescine, a base. Expression of these genes is controlled by pASR, an acid sensitive promoter sequence from the parts repository. The 2024 Oneonta iGEM team designed this sequence for use in pH adjusting devices for use in the remediation of wastewater from the manufacturing of microprocessors. | This composite part functions as an acid sensing, base producing part. It contains the coding sequences speA and speB, the two enzymes involved in the conversion of arginine to putrescine (Morris., et al 1969). These genes, when expressed, will produce putrescine, a base. Expression of these genes is controlled by pASR, an acid sensitive promoter sequence from the parts repository. The 2024 Oneonta iGEM team designed this sequence for use in pH adjusting devices for use in the remediation of wastewater from the manufacturing of microprocessors. | ||
| − | https://static.igem.wiki/teams/5097/parts/putrescine-pathway.jpg | + | ::::::::https://static.igem.wiki/teams/5097/parts/putrescine-pathway.jpg |
| − | ::Figure 1: Conversion of Arginine to Putrescine. Figure adapted from reference 2. | + | :::::Figure 1: Conversion of Arginine to Putrescine. Figure adapted from reference 2. |
| + | |||
| + | This part contains the following: | ||
| + | |||
| + | BBa K1231000 pASR | ||
| + | |||
| + | BBa_B0034 | ||
| + | |||
| + | BBa_K5097009 SpeA | ||
| + | |||
| + | BBa_B0034 | ||
| + | |||
| + | BBa_K5097010 SpeB | ||
===References=== | ===References=== | ||
Latest revision as of 13:28, 2 October 2024
Acid-sensor, Base-producer pASR-PUT1
Usage and Biology
This composite part functions as an acid sensing, base producing part. It contains the coding sequences speA and speB, the two enzymes involved in the conversion of arginine to putrescine (Morris., et al 1969). These genes, when expressed, will produce putrescine, a base. Expression of these genes is controlled by pASR, an acid sensitive promoter sequence from the parts repository. The 2024 Oneonta iGEM team designed this sequence for use in pH adjusting devices for use in the remediation of wastewater from the manufacturing of microprocessors.
- Figure 1: Conversion of Arginine to Putrescine. Figure adapted from reference 2.
This part contains the following:
BBa K1231000 pASR
BBa_B0034
BBa_K5097009 SpeA
BBa_B0034
BBa_K5097010 SpeB
References
1-Morris, D and Koffron, K. “Putrescine Biosynthesis in Escherichia coli: REGULATION THROUGH PATHWAY SELECTION” Journal of biological chemistry, vol. 244, 22, (1969): 6094-9. doi.org/10.1016/S0021-9258(18)63510-0.
2-Charlier, Daniel & Bervoets, Indra. (2019). Regulation of arginine biosynthesis, catabolism and transport in Escherichia coli. Amino Acids. 51. 10.1007/s00726-019-02757-8.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2930
Illegal BamHI site found at 1541
Illegal BamHI site found at 2843 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1319
Illegal AgeI site found at 2865 - 1000COMPATIBLE WITH RFC[1000]