Difference between revisions of "PBAD Promoter Family"

(Overview)
 
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=Overview=
 
=Overview=
 
[http://2009.igem.org/Team:British_Columbia We] have designed and characterized a family of arabinose-inducible pBAD promoters, consisting of the following members:
 
[http://2009.igem.org/Team:British_Columbia We] have designed and characterized a family of arabinose-inducible pBAD promoters, consisting of the following members:
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Note that a new set of pBAD promoters have been characterized; the [https://parts.igem.org/PBAD_SPL pBAD SPL] is a set of on non-leaky pBAD promoters with varying strength. 
 
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See the individual parts pages for details on characterization.
 
See the individual parts pages for details on characterization.
 
===Transfer Function===
 
===Transfer Function===
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[[Image:PBAD family - Transfer Function.png|center|frame|Transfer functions relating input concentration to output for each promoter in the pBAD family.]]
 
===Expression Rate===
 
===Expression Rate===
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[[Image:PBAD family - Timecourse.png|center|frame|Rate of RFP expression for each promoter in the pBAD family.]]
 
===Specificity===
 
===Specificity===
 
[[Image:PBAD family - Specificity.png|center|frame|All of the promoters in the pBAD family respond specifically to L-arabinose.]]
 
[[Image:PBAD family - Specificity.png|center|frame|All of the promoters in the pBAD family respond specifically to L-arabinose.]]

Latest revision as of 13:38, 4 October 2013

Family:        pBAD strong        pBAD wild type        pBAD weak       

Overview

[http://2009.igem.org/Team:British_Columbia We] have designed and characterized a family of arabinose-inducible pBAD promoters, consisting of the following members:

Note that a new set of pBAD promoters have been characterized; the pBAD SPL is a set of on non-leaky pBAD promoters with varying strength.

BBa_K206000: pBAD strong
BBa_I13453: pBAD wild type
BBa_K206001: pBAD weak
Alignment of the mutated bases in pBAD weak and pBAD strong compared to I13453.

Characterization

See the individual parts pages for details on characterization.

Transfer Function

Transfer functions relating input concentration to output for each promoter in the pBAD family.

Expression Rate

Rate of RFP expression for each promoter in the pBAD family.

Specificity

All of the promoters in the pBAD family respond specifically to L-arabinose.