Difference between revisions of "Part:BBa K187002:Experience"
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===Applications of BBa_K187002===
 
===Applications of BBa_K187002===
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HemF was one of two genes used to test the length of linear constructs that can be assembled with the Biobytes assembly method.
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All AB Bytes were hemF (900bp), and all BA Bytes were rplB (822bp). We started with the A anchor, then added AB hemF, followed by BA rplB. We alternated AB Bytes with BA Bytes until we had added 8 Bytes total (4 of each). Next, the construct was digested off the bead via USERTM mix. The construct was then run on a gel (Figure 1). We were able to assemble seven Bytes in a day.
  
 
https://static.igem.org/mediawiki/2009/f/f6/UofA_iGEM09_LinearConstruct1.png
 
https://static.igem.org/mediawiki/2009/f/f6/UofA_iGEM09_LinearConstruct1.png
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===User Reviews===
 
===User Reviews===
 
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Latest revision as of 00:48, 22 October 2009

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K187002

HemF was one of two genes used to test the length of linear constructs that can be assembled with the Biobytes assembly method.

All AB Bytes were hemF (900bp), and all BA Bytes were rplB (822bp). We started with the A anchor, then added AB hemF, followed by BA rplB. We alternated AB Bytes with BA Bytes until we had added 8 Bytes total (4 of each). Next, the construct was digested off the bead via USERTM mix. The construct was then run on a gel (Figure 1). We were able to assemble seven Bytes in a day.

UofA_iGEM09_LinearConstruct1.png

User Reviews

UNIQc900b798ea1f08e2-partinfo-00000000-QINU UNIQc900b798ea1f08e2-partinfo-00000001-QINU