Difference between revisions of "Part:BBa K5508007:Design"

 
 
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===Design Notes===
 
===Design Notes===
 
To verify the effectiveness of our protein expression system, we inserted the mCherry gene into the pESC-URA plasmid. Whether the protein expression system is effective or not can be determined by fluorescent expression or not.
 
To verify the effectiveness of our protein expression system, we inserted the mCherry gene into the pESC-URA plasmid. Whether the protein expression system is effective or not can be determined by fluorescent expression or not.
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    .bild {max-width: 35% ; height: auto;}
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  <img class="bild" src="https://static.igem.wiki/teams/5508/hangzhoumedx-parts/22.png">
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  <div class="unterschrift"><b> pESC-mCherry</b>
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  </div>
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Latest revision as of 20:57, 30 September 2024


Gal1,10-mCherry-CYC1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 374
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To verify the effectiveness of our protein expression system, we inserted the mCherry gene into the pESC-URA plasmid. Whether the protein expression system is effective or not can be determined by fluorescent expression or not.

pESC-mCherry


Source

None

References