Difference between revisions of "Part:BBa K5096070"

 
 
(One intermediate revision by one other user not shown)
Line 1: Line 1:
 +
__NOTOC__
 +
<partinfo>BBa_K5096070 short</partinfo>
 +
This sequence codes for the guide sequence of the sgRNA70. This sequence is derived from the inhA gene of Mycobacterium tuberculosis. Among M. tuberculosis genes, the inhA gene was selected due to its low mutation rate and its critical role in M. tuberculosis’s pathogenicity and survival (Marrakchi et al., 2014). By inhibiting inhA with our CRISPRi system, the sgRNA70 aims to disrupt M. tuberculosis's pathogenicity.
 +
This part is critical for locating the non-coding strand of the target DNA sequence, since it has complementary base pairs of inhA target.
  
 +
 +
<!-- Add more about the biology of this part here
 +
===Usage and Biology===
 +
 +
<!-- -->
 +
<span class='h3bb'>Sequence and Features</span>
 +
<partinfo>BBa_K5096070 SequenceAndFeatures</partinfo>
 +
 +
 +
<!-- Uncomment this to enable Functional Parameter display
 +
===Functional Parameters===
 +
<partinfo>BBa_K5096070 parameters</partinfo>
 +
<!-- -->

Latest revision as of 00:16, 2 October 2024

sg70 This sequence codes for the guide sequence of the sgRNA70. This sequence is derived from the inhA gene of Mycobacterium tuberculosis. Among M. tuberculosis genes, the inhA gene was selected due to its low mutation rate and its critical role in M. tuberculosis’s pathogenicity and survival (Marrakchi et al., 2014). By inhibiting inhA with our CRISPRi system, the sgRNA70 aims to disrupt M. tuberculosis's pathogenicity. This part is critical for locating the non-coding strand of the target DNA sequence, since it has complementary base pairs of inhA target.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]