Difference between revisions of "Part:BBa K5301020"

Latest revision as of 09:37, 1 October 2024

SnCSdT expression pathway (T7)

This pathway expresses SnCSdT using T7 expression system.

Usage and Biology

We used this pathway to express SnCSdT by using T7 expression system in E.coli, and the expression of SnCSdT can be induced by IPTG. For more information on SnCSdT, go to BBa_K5301006.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 1081
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1223
Illegal PstI site found at 1081
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 263
Illegal BglII site found at 635
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 1081
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 1081
Illegal AgeI site found at 160
Illegal AgeI site found at 920
1000
COMPATIBLE WITH RFC[1000]

@@ Line 7: / Line 7: @@
 ===Usage and Biology===
-We used this pathway to express SnCSdT by using T7 expression system in <i>E.coli</i>. And the expression of SnCSdT can be induced by IPTG. For more information on SnCSdT, go to <partinfo>BBa_K5301006</partinfo>.
+We used this pathway to express SnCSdT by using T7 expression system in <i>E.coli</i>, and the expression of SnCSdT can be induced by IPTG. For more information on SnCSdT, go to <partinfo>BBa_K5301006</partinfo>.
+===Sequence and Features===
 <!-- Add more about the biology of this part here
@@ Line 13: / Line 15: @@
 <!-- -->
-<span class='h3bb'>Sequence and Features</span>
+<span class='h3bb'></span>
 <partinfo>BBa_K5301020 SequenceAndFeatures</partinfo>