Difference between revisions of "Part:BBa K5322013:Design"

 
 
(One intermediate revision by the same user not shown)
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
To ensure the successful expression of SOD-1 in Escherichia coli, we performed codon optimization of the SOD-1 sequence.
+
To ensure the successful expression of SOD-1 in <i>Escherichia coli</i>, we performed codon optimization of the SOD-1 sequence.
  
  
  
 
===Source===
 
===Source===
−
 
+
<html>
−
The team obtains the SOD-1 sequence from BBa_K2215003.
+
The team obtains the SOD-1 sequence from <a href="https://parts.igem.org/Part:BBa_K2215003">BBa_K2215003</a>.
−
 
+
</html>
 
===References===
 
===References===

Latest revision as of 12:51, 1 October 2024


pET29a-J23119-RBS-SoxR-T7-pSoxS-RBS-(SOD-1)-T7.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 552
    Illegal NheI site found at 1157
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To ensure the successful expression of SOD-1 in Escherichia coli, we performed codon optimization of the SOD-1 sequence.


Source

The team obtains the SOD-1 sequence from BBa_K2215003.

References