Difference between revisions of "Part:BBa K5301023"

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===Usage and Biology===
 
===Usage and Biology===
  
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We used this pathway to express SCSdC-mCh[1-10] by using T7 expression system in <i>E.coli</i>. And the expression of SCSdC-mCh[1-10] can be induced by IPTG. For more information on SCSdC-mCh[1-10], go to <partinfo>BBa_K5301005</partinfo>.
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We used this pathway to express SCSdC-mCh[1-10] by using T7 expression system in <i>E.coli</i>, and the expression of SCSdC-mCh[1-10] can be induced by IPTG. For more information on SCSdC-mCh[1-10], go to <partinfo>BBa_K5301005</partinfo>.
  
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===Characterization===  
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===Sequence and Features===
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We used SDS-PAGE to test whether this pathway is expressed successfully(Figure 1). The molecular weight of SCSdC-mCh[1-10] is 76.3 kDa. And we purified the target protein with a molecular weight of approximately 70-100 kD (Lane 4 - Lane 5), which demonstrated that we had successfully expressed this pathway.
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<div class="center"><div class="thumb tnone"><div class="thumbinner" style="width:min-content;"><div style="zoom:0.5;overflow:hidden;">
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https://static.igem.wiki/teams/5301/parts/sds-page-result-of-sdycatcher-for-sdycatcher.png
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</div><div class="thumbcaption">
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Figure 1.SDS-PAGE analysis of the express results of SCSdC-mCh[1-10] (containing SdyCatcher).The gel was run at 80 V for 10 minutes and then at 150 V for 20 minutes, followed by staining with Coomassie Brilliant Blue. The molecular weight of SCSdC-mCh[1-10] is 76.3 kDa.
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K5301023 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K5301023 SequenceAndFeatures</partinfo>
  

Latest revision as of 09:36, 1 October 2024


This pathway expresses SCSdC-mCh[1-10] using T7 expression system.

This pathway expresses SCSdC-mCh[1-10] using T7 expression system.

Usage and Biology

We used this pathway to express SCSdC-mCh[1-10] by using T7 expression system in E.coli, and the expression of SCSdC-mCh[1-10] can be induced by IPTG. For more information on SCSdC-mCh[1-10], go to BBa_K5301005.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1764
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1283
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1079
    Illegal AgeI site found at 1872
  • 1000
    COMPATIBLE WITH RFC[1000]