Difference between revisions of "Part:BBa K5301023"
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===Usage and Biology=== | ===Usage and Biology=== | ||
− | We used this pathway to express SCSdC-mCh[1-10] by using T7 expression system in <i>E.coli</i> | + | We used this pathway to express SCSdC-mCh[1-10] by using T7 expression system in <i>E.coli</i>, and the expression of SCSdC-mCh[1-10] can be induced by IPTG. For more information on SCSdC-mCh[1-10], go to <partinfo>BBa_K5301005</partinfo>. |
− | === | + | ===Sequence and Features=== |
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Latest revision as of 09:36, 1 October 2024
This pathway expresses SCSdC-mCh[1-10] using T7 expression system.
This pathway expresses SCSdC-mCh[1-10] using T7 expression system.
Usage and Biology
We used this pathway to express SCSdC-mCh[1-10] by using T7 expression system in E.coli, and the expression of SCSdC-mCh[1-10] can be induced by IPTG. For more information on SCSdC-mCh[1-10], go to BBa_K5301005.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 1764
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1283
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1079
Illegal AgeI site found at 1872 - 1000COMPATIBLE WITH RFC[1000]