Difference between revisions of "Part:BBa K243003"

Latest revision as of 03:02, 22 October 2009

Digoxigenin binding protein (DigA)

This part is a synthetic protein from the anticalin type which is derived from the naturally existing protein family of lipocalins. Like other antibody formats, it is able to bind an antigen. In case of this protein, the antigen is digoxigenin which can be present on labeled synthetic oligonucleotides.

Usage and Biology

Normally a digoxigenin antibody is used for nonradioactive in situ hybridization.
We used this part to connect our universal restriction endonuclease protein domains to digoxigenin labeled oligos. These oligos were hybridized with ssDNA and so created a connection between the protein and the DNA. Another possiblity is to use TFO's (triplex-forming oligonucleotides) labeled with digoxigenin to establish a connection between dsDNA and protein.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 248
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K243003 short</partinfo> <br>
-This part is a synthetic protein called "anticaline" which is derived from the naturally existing protein lipocaline. It is able to bind antigen and therefore functions as a linker to another part/protein, which is labeled with digoxigenin.
+This part is a synthetic protein from the anticalin type which is derived from the naturally existing protein family of lipocalins. Like other antibody formats, it is able to bind an antigen. In case of this protein, the antigen is digoxigenin which can be present on labeled synthetic oligonucleotides.
 ===Usage and Biology===
-Normally a digoxigenin antibody is used for nonradioactive In Situ Hybridization.<br>
+Normally a digoxigenin antibody is used for nonradioactive in situ hybridization.<br>
-We used this part to connect our universal restriction endonuclease protein domains to digoxigenin labeled oligos. These oligos were hybridized with ssDNA and so they made a connection between the protein and the DNA. Another possiblity is to use TFO's (triplex-forming oligonucleotides) labeled with digoxigenin to establish a connection between dsDNA and protein.<br> <br>
+We used this part to connect our universal restriction endonuclease protein domains to digoxigenin labeled oligos. These oligos were hybridized with ssDNA and so created a connection between the protein and the DNA. Another possiblity is to use TFO's (triplex-forming oligonucleotides) labeled with digoxigenin to establish a connection between dsDNA and protein.<br> <br>
 [[Image:Dig2b.JPG|overview of immunohistochemical applications]]<br>
 <br>