Difference between revisions of "Part:BBa K5047003"

<!-- This DNA sequence encodes a shRNA which can be used as a control in RNA interference experiments. Instead of targeting a functional gene this sequence contains two inverted repeats of a sgRNA derived from a CRISPR interference system separated by a 9nt loop Brummelkamp Thijn R et al A system for stable expression of short interfering RNAs in mammalian cells Science New York NY vol 2965567 2002 550-3 doi1061126science1068999. The shRNA does not have any significant biological target in our model organism making it an ideal negative control to verify the specificity and functionality of the RNA interference RNAi machinery in our experiments. This ensures that any observed effects in the system are due to the specific shRNAs being tested and not due to nonspecific or off-target interactions.