Difference between revisions of "Part:BBa K5097008"
 
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The 2024 Oneonta iGEM team pHish and CHIPs created this acid-sensitive reporter circuit to characterize the expression of genes in response to acidic conditions, using pASR (BBa_K1231000). To do this, we used pARS to drive the expression of RFP, a reporter gene.   
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The 2024 Oneonta iGEM team pHish and CHIPs created this acid-sensitive reporter circuit to characterize the expression of genes in response to acidic conditions, using pASR (BBa_K1231000). To do this, we used pASR to drive the expression of RFP, a reporter gene.   
  
 
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Latest revision as of 14:58, 30 September 2024


pASR-RFP reporter

Usage and Biology

The 2024 Oneonta iGEM team pHish and CHIPs created this acid-sensitive reporter circuit to characterize the expression of genes in response to acidic conditions, using pASR (BBa_K1231000). To do this, we used pASR to drive the expression of RFP, a reporter gene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 707
    Illegal AgeI site found at 819
  • 1000
    COMPATIBLE WITH RFC[1000]