Difference between revisions of "Part:BBa K5136042"
 
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<partinfo>BBa_K5136042 short</partinfo>
 
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===Biology===
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LexRO is a synthetic light-switchable repressor, based on a novel LOV light sensor domain, RsLOV. In the darkness, LexRO dimerizes and binds to its cognate operator sequence to repress promoter activity. Upon light exposure, the LexRO dimer dissociates, causing dissociation from the operator sequence, and initiates gene expression.
  
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===Usage and design===
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===Usage and Biology===
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In the darkness, LexRO dimerizes and binds to the cognate operator sequence to repress the activity of pColE408. Upon blue light exposure, the LexRO dimer dissociates, causing dissociation from the operator sequence, and initiates the expression of ccdB， eventually leading to cell death. We used LexRO, pHybrid 2)-114 version, SD7, ccdA, and ccdB to construct the regulation system and obtained the composite part <partinfo>BBa_K5136231</partinfo>, which was assembled on the expression vector pSB4A5.
  
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<center><html><img src="https://static.igem.wiki/teams/4907/wiki/parts/haoxihuan/and-gate.png" width="700px"></html></center>
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<center><b>Fig. 1 Gene circuits of verification system for <i>hrp</i> AND gate. </b></center>
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===Characterization===
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====Agarose gel electrophoresis (AGE)====
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When we were building this circuit, colony PCR was used to certify the plasmid was correct. We got the target fragment-****bp(Fig. 3).
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<center><html><img src="https://static.igem.wiki/teams/4907/wiki/parts/haoxihuan/cspa-and-gate.png" width="700px"></html></center>
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<center><b>Fig. 2 Gene circuits of <i>hrp</i> system regulated by the CspA CRE.</b></center>
 
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<span class='h3bb'>LexRO is a synthetic light-switchable repressor, based on a novel LOV light sensor domain, RsLOV. In the darkness, LexRO dimerizes and binds to its cognate operator sequence to repress promoter activity. Upon light exposure, the LexRO dimer dissociates, causing dissociation from the operator sequence, and initiates gene expression.</span>
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<span class='h3bb'>Sequence and Features</span>
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<span class='h3bb'>In the darkness, LexRO dimerizes and binds to the cognate operator sequence to repress the activity of pColE408. Upon blue light exposure, the LexRO dimer dissociates, causing dissociation from the operator sequence, and initiates the expression of ccdB， eventually leading to cell death. We used LexRO, pHybrid 2)-114 version, SD7, ccdA, and ccdB to construct the regulation system and obtained the composite part BBa K5136231 , which was assembled on the expression vector pSB4A5.</span>
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<partinfo>BBa_K5136042 SequenceAndFeatures</partinfo>
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Latest revision as of 11:18, 30 September 2024


LexRO

Biology

LexRO is a synthetic light-switchable repressor, based on a novel LOV light sensor domain, RsLOV. In the darkness, LexRO dimerizes and binds to its cognate operator sequence to repress promoter activity. Upon light exposure, the LexRO dimer dissociates, causing dissociation from the operator sequence, and initiates gene expression.

Usage and design

In the darkness, LexRO dimerizes and binds to the cognate operator sequence to repress the activity of pColE408. Upon blue light exposure, the LexRO dimer dissociates, causing dissociation from the operator sequence, and initiates the expression of ccdB， eventually leading to cell death. We used LexRO, pHybrid 2)-114 version, SD7, ccdA, and ccdB to construct the regulation system and obtained the composite part BBa_K5136231, which was assembled on the expression vector pSB4A5.

Fig. 1 Gene circuits of verification system for hrp AND gate.

Characterization

Agarose gel electrophoresis (AGE)

When we were building this circuit, colony PCR was used to certify the plasmid was correct. We got the target fragment-****bp(Fig. 3).

Fig. 2 Gene circuits of hrp system regulated by the CspA CRE.

Sequence and Features