Difference between revisions of "Part:BBa K5382110"
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<partinfo>BBa_K5382110 short</partinfo> | <partinfo>BBa_K5382110 short</partinfo> | ||
| − | InaK is a kind of ice nucleoprotein of <i>Pseudomonas syringae</i> KCTC1832. Ice nucleoprotein is a kind of protein that can promote the formation of ice crystals in organisms. Its N-terminal domain can be tightly anchored to the surface of <i> | + | The InaK is a kind of ice nucleoprotein of <i>Pseudomonas syringae</i> KCTC1832. Ice nucleoprotein is a kind of protein that can promote the formation of ice crystals in organisms. Its N-terminal domain can be tightly anchored to the surface of <i>Escherichia coli</i> Nissle1917 cells as an anchor moda, so as to enable surface display.<br> |
| − | linker here is a short peptide used to connect the InaK and z domain.<br> | + | The linker here is a short peptide used to connect the InaK and z domain.<br> |
| − | z domain refers to a synthetic protein domain that has the ability to bind to the Fc region of immunoglobulin G (IgG)[1]. In our project, z domain is used to construct extracellular vesicles (EVs) that can display antibodies, so that these EVs can bind specific antibodies through the z domain on their surface, so as to achieve targeted delivery to specific cells or tissues | + | The z domain refers to a synthetic protein domain that has the ability to bind to the Fc region of immunoglobulin G (IgG)<sup>[1]</sup>. In our project, the z domain is used to construct extracellular vesicles (EVs) that can display antibodies, so that these EVs can bind specific antibodies through the z domain on their surface, so as to achieve targeted delivery to specific cells or tissues<Br> |
| − | + | ===References=== | |
| + | [1] Oscar P B Wiklander;Doste R Mamand;Dara K Mohammad;Wenyi Zheng;Rim Jawad Wiklander;Taras Sych;Antje M Zickler;Xiuming Liang;Heena Sharma;Andrea Lavado;Jeremy Bost;Samantha Roudi;Giulia Corso;Angus J Lennaárd;Manuchehr Abedi Valugerdi;Imre Mäger;Evren Alici;Erdinc Sezgin;Joel Z Nordin;Dhanu Gupta;André Görgens;Samir El Andaloussi.Antibody-displaying extracellular vesicles for targeted cancer therapy.[J].Nature biomedical engineering,2024,() | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Latest revision as of 10:20, 1 October 2024
Inak-linker-z domain_protein domain that have the ability to bind to lgG
The InaK is a kind of ice nucleoprotein of Pseudomonas syringae KCTC1832. Ice nucleoprotein is a kind of protein that can promote the formation of ice crystals in organisms. Its N-terminal domain can be tightly anchored to the surface of Escherichia coli Nissle1917 cells as an anchor moda, so as to enable surface display.
The linker here is a short peptide used to connect the InaK and z domain.
The z domain refers to a synthetic protein domain that has the ability to bind to the Fc region of immunoglobulin G (IgG)[1]. In our project, the z domain is used to construct extracellular vesicles (EVs) that can display antibodies, so that these EVs can bind specific antibodies through the z domain on their surface, so as to achieve targeted delivery to specific cells or tissues
References
[1] Oscar P B Wiklander;Doste R Mamand;Dara K Mohammad;Wenyi Zheng;Rim Jawad Wiklander;Taras Sych;Antje M Zickler;Xiuming Liang;Heena Sharma;Andrea Lavado;Jeremy Bost;Samantha Roudi;Giulia Corso;Angus J Lennaárd;Manuchehr Abedi Valugerdi;Imre Mäger;Evren Alici;Erdinc Sezgin;Joel Z Nordin;Dhanu Gupta;André Görgens;Samir El Andaloussi.Antibody-displaying extracellular vesicles for targeted cancer therapy.[J].Nature biomedical engineering,2024,() Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1674
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 722
Illegal NgoMIV site found at 962
Illegal NgoMIV site found at 1130
Illegal AgeI site found at 517 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 692
Illegal BsaI.rc site found at 1182
Illegal SapI.rc site found at 326