Difference between revisions of "Part:BBa K5415003:Design"

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__NOTOC__
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<partinfo>BBa_K5415003 short</partinfo>
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<partinfo>BBa_K5415003 SequenceAndFeatures</partinfo>
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<h5>Three sets of triple HRE containging oligonucleotide from EP0 promoter:</h5>
 
<h5>Three sets of triple HRE containging oligonucleotide from EP0 promoter:</h5>
 
<p>TCGACAAGCTTGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAC</p>
 
<p>TCGACAAGCTTGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAC</p>

Latest revision as of 11:40, 30 September 2024

Nonuple HRE biosensor with RFP for severe hypoxia detection.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1
    Illegal XhoI site found at 21
    Illegal XhoI site found at 35
    Illegal XhoI site found at 55
    Illegal XhoI site found at 69
    Illegal XhoI site found at 89
    Illegal XhoI site found at 103
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]
Three sets of triple HRE containging oligonucleotide from EP0 promoter:

TCGACAAGCTTGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAC

TCGACAAGCTTGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAC

TCGACAAGCTTGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAC

Post EcoRV CUT:

gat

CYC1 promoter from pRL_yEGFP_hc:

gcatgtgctctgtatgtatataaaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgcagcataaattactatacttctat

yeCherry:

ATGGTAAGCAAAGGTGAAGAAGATAACATGGCTATTATAAAAGAgTTCATGAGATTTAAGGTTCATATGGAAGGTTCTGTTAACGGGCATGAgTTCGAAATTGAA

GGTGAAGGTGAAGGTAGACCTTATGAAGGTACACAAACAGCTAAGTTAAAGGTCACAAAAGGTGGTCCCTTGCCATTCGCTTGGGACATTTTGTCTCCACAATTT

ATGTACGGTAGCAAGGCATACGTCAAGCACCCAGCTGACATTCCAGATTATTTGAAATTATCTTTTCCTGAAGGGTTTAAATGGGAAAGAGTTATGAATTTTGAG

GATGGTGGTGTTGTCACTGTCACACAAGACTCCAGTTTACAAGATGGTGAGTTCATCTACAAGGTTAAATTGAGAGGTACTAACTTTCCATCAGACGGTCCTGTT

ATGCAAAAGAAAACTATGGGTTGGGAAGCTAGCTCCGAAAGAATGTATCCTGAAGATGGTGCTTTGAAGGGTGAAATTAAGCAAAGATTGAAATTGAAAGATGGT

GGTCATTATGATGCAGAAGTTAAGACAACGTATAAAGCGAAAAAACCAGTTCAGCTTCCAGGTGCTTATAATGTTAATATTAAGTTGGACATTACCTCTCATAAT

GAGGACTACACTATTGTTGAACAATATGAAAGAGCTGAAGGCAGACATTCTACTGGTGGTATGGATGAATTGTACAAGTAA

ADH2 terminator:

gcggatctcttatgtctttacgatttatagttttcattatcaagtatgcctatattagtatatagcatctttagatgacagtgttcgaagtttcacgaataaaagataata

ttctactttttgctcccaccgcgtttgctagcacgagtgaacaccatccctcgcctgtgagttgtacccattcctctaaactgtagacatggtagcttcagcagtgttcgt

tatgtacggcatcctccaacaaacagtcggttatagtttgtcctgctcctctgaatcgtatccctcgatatttctcat