Difference between revisions of "Part:BBa K5415003:Design"

Latest revision as of 11:40, 30 September 2024

Nonuple HRE biosensor with RFP for severe hypoxia detection.

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 1
Illegal XhoI site found at 21
Illegal XhoI site found at 35
Illegal XhoI site found at 55
Illegal XhoI site found at 69
Illegal XhoI site found at 89
Illegal XhoI site found at 103
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Three sets of triple HRE containging oligonucleotide from EP0 promoter:

TCGACAAGCTTGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAC

Post EcoRV CUT:

gat

CYC1 promoter from pRL_yEGFP_hc:

gcatgtgctctgtatgtatataaaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgcagcataaattactatacttctat

yeCherry:

ATGGTAAGCAAAGGTGAAGAAGATAACATGGCTATTATAAAAGAgTTCATGAGATTTAAGGTTCATATGGAAGGTTCTGTTAACGGGCATGAgTTCGAAATTGAA

GGTGAAGGTGAAGGTAGACCTTATGAAGGTACACAAACAGCTAAGTTAAAGGTCACAAAAGGTGGTCCCTTGCCATTCGCTTGGGACATTTTGTCTCCACAATTT

ATGTACGGTAGCAAGGCATACGTCAAGCACCCAGCTGACATTCCAGATTATTTGAAATTATCTTTTCCTGAAGGGTTTAAATGGGAAAGAGTTATGAATTTTGAG

GATGGTGGTGTTGTCACTGTCACACAAGACTCCAGTTTACAAGATGGTGAGTTCATCTACAAGGTTAAATTGAGAGGTACTAACTTTCCATCAGACGGTCCTGTT

ATGCAAAAGAAAACTATGGGTTGGGAAGCTAGCTCCGAAAGAATGTATCCTGAAGATGGTGCTTTGAAGGGTGAAATTAAGCAAAGATTGAAATTGAAAGATGGT

GGTCATTATGATGCAGAAGTTAAGACAACGTATAAAGCGAAAAAACCAGTTCAGCTTCCAGGTGCTTATAATGTTAATATTAAGTTGGACATTACCTCTCATAAT

GAGGACTACACTATTGTTGAACAATATGAAAGAGCTGAAGGCAGACATTCTACTGGTGGTATGGATGAATTGTACAAGTAA

ADH2 terminator:

gcggatctcttatgtctttacgatttatagttttcattatcaagtatgcctatattagtatatagcatctttagatgacagtgttcgaagtttcacgaataaaagataata

ttctactttttgctcccaccgcgtttgctagcacgagtgaacaccatccctcgcctgtgagttgtacccattcctctaaactgtagacatggtagcttcagcagtgttcgt

tatgtacggcatcctccaacaaacagtcggttatagtttgtcctgctcctctgaatcgtatccctcgatatttctcat

@@ Line 1: / Line 1: @@
-<h5>Three sets of triple HRE containging oligonucleotide from EP0 promoter</h5>
+__NOTOC__
+<partinfo>BBa_K5415003 short</partinfo>
+<partinfo>BBa_K5415003 SequenceAndFeatures</partinfo>
+<h5>Three sets of triple HRE containging oligonucleotide from EP0 promoter:</h5>
 <p>TCGACAAGCTTGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAC</p>
 <p>TCGACAAGCTTGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAC</p>
 <p>TCGACAAGCTTGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAC</p>
 <p></p>
-<h5>Post EcoRV CUT</h5>
+<h5>Post EcoRV CUT:</h5>
 <p>gat</p>
 <p></p>
-<h5>CYC1 promoter from pRL_yEGFP_hc</h5>
+<h5>CYC1 promoter from pRL_yEGFP_hc:</h5>
 <p>gcatgtgctctgtatgtatataaaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgcagcataaattactatacttctat</p>
 <p></p>
-<h5>yeCherry</h5>
+<h5>yeCherry:</h5>
 <p>ATGGTAAGCAAAGGTGAAGAAGATAACATGGCTATTATAAAAGAgTTCATGAGATTTAAGGTTCATATGGAAGGTTCTGTTAACGGGCATGAgTTCGAAATTGAA</p>
 <p>GGTGAAGGTGAAGGTAGACCTTATGAAGGTACACAAACAGCTAAGTTAAAGGTCACAAAAGGTGGTCCCTTGCCATTCGCTTGGGACATTTTGTCTCCACAATTT</p>
@@ Line 19: / Line 24: @@
 <p>GAGGACTACACTATTGTTGAACAATATGAAAGAGCTGAAGGCAGACATTCTACTGGTGGTATGGATGAATTGTACAAGTAA</p>
 <p></p>
-<h5>ADH2 terminator</h5>
+<h5>ADH2 terminator:</h5>
 <p>gcggatctcttatgtctttacgatttatagttttcattatcaagtatgcctatattagtatatagcatctttagatgacagtgttcgaagtttcacgaataaaagataata</p>
 <p>ttctactttttgctcccaccgcgtttgctagcacgagtgaacaccatccctcgcctgtgagttgtacccattcctctaaactgtagacatggtagcttcagcagtgttcgt</p>
 <p>tatgtacggcatcctccaacaaacagtcggttatagtttgtcctgctcctctgaatcgtatccctcgatatttctcat</p>