Difference between revisions of "Part:BBa K5207011"
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<figcaption><center>Figure 1. Snapgene diagrams of pYTK095</center></figcaption> | <figcaption><center>Figure 1. Snapgene diagrams of pYTK095</center></figcaption> | ||
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<figcaption><center>Figure 2. Fluorescent labeling screening of pYTK095</center></figcaption> | <figcaption><center>Figure 2. Fluorescent labeling screening of pYTK095</center></figcaption> | ||
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<figcaption><center>Figure 3. Validation plot of pYTK095 gel electrophoresis</center></figcaption> | <figcaption><center>Figure 3. Validation plot of pYTK095 gel electrophoresis</center></figcaption> | ||
Latest revision as of 13:21, 30 September 2024
pCCW12-SmCPS1-tSSA1
This is an expression cassette consisting of a promoter pCCW12, the SmCPS1 gene, and a terminator tSSA1.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 2397
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1158
Illegal BamHI site found at 756
Illegal XhoI site found at 892
Illegal XhoI site found at 916
Illegal XhoI site found at 2209
Illegal XhoI site found at 2419
Illegal XhoI site found at 2891 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 561
Illegal NgoMIV site found at 1978
Illegal NgoMIV site found at 2542 - 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
We combined each Level 0 junction module, promoter, CDS, terminator, and ending module, and constructed them into vector pYTK095 through BsaI digestion and ligation, and this step was the construction of Level 1.
After transformation in E. coli DH10B, we selected the single bacterial colony (pYTK095) that does not fluoresce under UV light from the Petri dishes. It was used for colony PCR amplification and validation.
