Difference between revisions of "Part:BBa K5079016"
 
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<partinfo>BBa_K5079016 short</partinfo>
 
<partinfo>BBa_K5079016 short</partinfo>
  
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This is the reverse primer for Part:BBa_K5079033. This is cloned in frame with the his tag, primers were used with the q5 polymerase master mix. The sequence is: GGTGCTCGAGTGCGGCCGCAGA TGAcaactggccacagtc. 3’ GC Content is 56%, 3' length is 18nt and 3' Tm is 65°C.
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This part is the reverse primer for switch 8 (K45_CP_N114, part BBa_K5079033) into pET-28a. It allows for the addition of overhangs to the sequence of protein switch 8 that are complementary to the pET-28a sequence for cloning. It has a melting point of 65.0°C . Part "BBa_ K5079015" used as the forward primer. Cloned in frame with a His Tag. Used with q5 polymerase mastermix.
  
  

Latest revision as of 01:52, 1 October 2024


K45_CP_N114 Switch primers into pET-28a Switch 8 (Rev)

This part is the reverse primer for switch 8 (K45_CP_N114, part BBa_K5079033) into pET-28a. It allows for the addition of overhangs to the sequence of protein switch 8 that are complementary to the pET-28a sequence for cloning. It has a melting point of 65.0°C . Part "BBa_ K5079015" used as the forward primer. Cloned in frame with a His Tag. Used with q5 polymerase mastermix.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 12
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 5
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]