Difference between revisions of "Part:BBa K5079013"
 
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<partinfo>BBa_K5079013 short</partinfo>
 
<partinfo>BBa_K5079013 short</partinfo>
  
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This is the forward primer for Part:BBa_K5079032. This is cloned in frame with the his tag, primers were used with the q5 polymerase master mix. The sequence is: ACTTTAAGAAGGAGATATAC atgtcgggaaatcccttgg. 3’ GC Content is 61%, 3' length is 18nt and 3' Tm is 68°C.
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This is the forward primer for Part:BBa_K5079032. This is cloned in frame with the his tag, primers were used with the q5 polymerase master mix. The sequence is: ACTTTAAGAAGGAGATATAC atgtcgggaaatcccttgg. 3’ GC Content is 53%, 3' length is 19nt and 3' Tm is 65°C.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 08:59, 30 September 2024


K45_CP_P95 Switch primers into pET-28a Switch 7 (Fwd)

This is the forward primer for Part:BBa_K5079032. This is cloned in frame with the his tag, primers were used with the q5 polymerase master mix. The sequence is: ACTTTAAGAAGGAGATATAC atgtcgggaaatcccttgg. 3’ GC Content is 53%, 3' length is 19nt and 3' Tm is 65°C.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]