Difference between revisions of "Part:BBa K5415002:Design"

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<h5>Two sets of triple HRE containing oligonucleotide from EP0 promoter</h5>
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__NOTOC__
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<partinfo>BBa_K5415002 short</partinfo>
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 +
<partinfo>BBa_K5415002 SequenceAndFeatures</partinfo>
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 +
<h5>Two sets of triple HRE containing oligonucleotide from EP0 promoter:</h5>
 
<p>TCGACAAGCTTGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAC</p>
 
<p>TCGACAAGCTTGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAC</p>
 
<p>TCGACAAGCTTGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAC</p>
 
<p>TCGACAAGCTTGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAC</p>
 
<p></p>
 
<p></p>
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<h5>Post-EcoRV CUT</h5>
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<h5>Post-EcoRV CUT:</h5>
 
<p>gat</p>
 
<p>gat</p>
 
<p></p>
 
<p></p>
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<h5>CYC1 promoter from pRL_yEGFP_hc</h5>
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<h5>CYC1 promoter from pRL_yEGFP_hc:</h5>
 
<p>gcatgtgctctgtatgtatataaaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgcagcataaattactatacttctat</p>
 
<p>gcatgtgctctgtatgtatataaaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgcagcataaattactatacttctat</p>
 
<p></p>
 
<p></p>
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<h5>yeBFP2</h5>
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<h5>yeBFP2:</h5>
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<p>ATGGTGTCAAAAGGTGAAGAATTGTTCACAGGTGTTGTTCCAATTCTAGTTGAATTAGATGGTGACGTGAATGGTCATAAGTTTTCAGTTAGAGGTGAAGGTGA</p>
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<p>ATGGTGTCAAAAGGTGAAGAATTGTTCACAGGTGTTGTTCCAATTCTAGTTGAATTAGATGGTGACGTGAATGGTCATAAGTTTTCAGTTAGAGGTGAAGGT</p>
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<p>AGGTGATGCAACAAATGGTAA<p>ATTGACATTGAAGTTTATTTGTACAACCGGTAAGTTGCCAGTTCCGTGGCCAACTCTAGTTACTACATTGTCTCATGGGG</p>
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<p>GAAGGTGATGCAACAAATGGTAAATTGACATTGAAGTTTATTTGTACAACCGGTAAGTTGCCAGTTCCGTGGCCAACTCTAGTTACTACATTGTCTCATGGG</p>
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<p>TTCAGTGCTTTGCCAGATATCCAGATCACATGAAACAACATGATT<p>TCTTCAAATCTGCTATGCCAGAAGGTTATGTTCAAGAAAGAACCATTTTTTTTAAA</p>
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<p>GTTCAGTGCTTTGCCAGATATCCAGATCACATGAAACAACATGATTTCTTCAAATCTGCTATGCCAGAAGGTTATGTTCAAGAAAGAACCATTTTTTTTAAA</p>
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<p>GATGACGGTACATACAAAACAAGAGCTGAAGTTAAGTTCGAAGGTGATACTTTGGTTAATCGCATAGAA<p>TTGAAGGGCGTTGATTTTAAGGAAGATGGTAA</p>
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<p>GATGACGGTACATACAAAACAAGAGCTGAAGTTAAGTTCGAAGGTGATACTTTGGTTAATCGCATAGAATTGAAGGGCGTTGATTTTAAGGAAGATGGTAAC</p>
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<p>CATCCTCGGTCATAAGCTAGAATACAATTTCAATTCTCATAATATCTATATTATGGCTGTTAAACAAAAGAATGGTATTAAGGTTAATTTCAA<p>AATTAGAC</p>
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<p>ATCCTCGGTCATAAGCTAGAATACAATTTCAATTCTCATAATATCTATATTATGGCTGTTAAACAAAAGAATGGTATTAAGGTTAATTTCAAAATTAGACAT</p>
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<p>ATAATGTCGAAGATGGTTCAGTCCAATTAGCTGATCACTATCAACAAAATACCCCTATTGGTGATGGTCCTGTTTTATTGCCCGATTCTCATTATTTGTCAACT</p>
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<p>AATGTCGAAGATGGTTCAGTCCAATTAGCTGATCACTATCAACAAAATACCCCTATTGGTGATGGTCCTGTTTTATTGCCCGATTCTCATTATTTGTCAACT</p>
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<p>CAATCTGTTTTAT<p>CAAAAGATCCAAACGAAAAACGTGATCATATGGTTTTGTTGGAgTTCAGAACTGCCGCAGGAATTACTTTAGGTATGGATGAACTATATAAGTAG</p>
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<p>CAATCTGTTTTATCAAAAGATCCAAACGAAAAACGTGATCATATGGTTTTGTTGGAgTTCAGAACTGCCGCAGGAATTACTTTAGGTATGGATGAACTATAT</p>
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<p>AAGTAG</p>
 +
TAAGTAG</p>
 
<p></p>
 
<p></p>
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<h5>ADH2 terminator</h5>
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<h5>ADH2 terminator:</h5>
 
<p>gcggatctcttatgtctttacgatttatagttttcattatcaagtatgcctatattagtatatagcatctttagatgacagtgttcgaagtttcacgaataaaag</p>
 
<p>gcggatctcttatgtctttacgatttatagttttcattatcaagtatgcctatattagtatatagcatctttagatgacagtgttcgaagtttcacgaataaaag</p>
 
<p>ataatattctactttttgctcccaccgcgtttgctagcacgagtgaacaccatccctcgcctgtgagttgtacccattcctctaaactgtagacatggtagcttc</p>
 
<p>ataatattctactttttgctcccaccgcgtttgctagcacgagtgaacaccatccctcgcctgtgagttgtacccattcctctaaactgtagacatggtagcttc</p>
 
<p>agcagtgttcgttatgtacggcatcctccaacaaacagtcggttatagtttgtcctgctcctctgaatcgtatccctcgatatttctcat</p>
 
<p>agcagtgttcgttatgtacggcatcctccaacaaacagtcggttatagtttgtcctgctcctctgaatcgtatccctcgatatttctcat</p>

Latest revision as of 11:39, 30 September 2024

Hexuple HRE biosensor with BFP for moderate hypoxia detection.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1
    Illegal XhoI site found at 21
    Illegal XhoI site found at 35
    Illegal XhoI site found at 55
    Illegal XhoI site found at 69
    Illegal XhoI site found at 89
    Illegal XhoI site found at 103
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]
Two sets of triple HRE containing oligonucleotide from EP0 promoter:

TCGACAAGCTTGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAC

TCGACAAGCTTGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAC

Post-EcoRV CUT:

gat

CYC1 promoter from pRL_yEGFP_hc:

gcatgtgctctgtatgtatataaaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgcagcataaattactatacttctat

yeBFP2:

ATGGTGTCAAAAGGTGAAGAATTGTTCACAGGTGTTGTTCCAATTCTAGTTGAATTAGATGGTGACGTGAATGGTCATAAGTTTTCAGTTAGAGGTGAAGGT

GAAGGTGATGCAACAAATGGTAAATTGACATTGAAGTTTATTTGTACAACCGGTAAGTTGCCAGTTCCGTGGCCAACTCTAGTTACTACATTGTCTCATGGG

GTTCAGTGCTTTGCCAGATATCCAGATCACATGAAACAACATGATTTCTTCAAATCTGCTATGCCAGAAGGTTATGTTCAAGAAAGAACCATTTTTTTTAAA

GATGACGGTACATACAAAACAAGAGCTGAAGTTAAGTTCGAAGGTGATACTTTGGTTAATCGCATAGAATTGAAGGGCGTTGATTTTAAGGAAGATGGTAAC

ATCCTCGGTCATAAGCTAGAATACAATTTCAATTCTCATAATATCTATATTATGGCTGTTAAACAAAAGAATGGTATTAAGGTTAATTTCAAAATTAGACAT

AATGTCGAAGATGGTTCAGTCCAATTAGCTGATCACTATCAACAAAATACCCCTATTGGTGATGGTCCTGTTTTATTGCCCGATTCTCATTATTTGTCAACT

CAATCTGTTTTATCAAAAGATCCAAACGAAAAACGTGATCATATGGTTTTGTTGGAgTTCAGAACTGCCGCAGGAATTACTTTAGGTATGGATGAACTATAT

AAGTAG

TAAGTAG</p>

ADH2 terminator:

gcggatctcttatgtctttacgatttatagttttcattatcaagtatgcctatattagtatatagcatctttagatgacagtgttcgaagtttcacgaataaaag

ataatattctactttttgctcccaccgcgtttgctagcacgagtgaacaccatccctcgcctgtgagttgtacccattcctctaaactgtagacatggtagcttc

agcagtgttcgttatgtacggcatcctccaacaaacagtcggttatagtttgtcctgctcctctgaatcgtatccctcgatatttctcat