Difference between revisions of "Part:BBa K5117021"
 
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This part contains the regulatory sequence of P<sub><i>cotYZ</i></sub>, which is a promoter of <i>Bacillus subtilis</i>.  
 
This part contains the regulatory sequence of P<sub><i>cotYZ</i></sub>, which is a promoter of <i>Bacillus subtilis</i>.  
 
P<sub><i>cotYZ</i></sub> is a sporulation-dependent promoter out of the <i>cotYZ</i> gene cluster of <i>B. subtilis</i>, which allows for gene expression only during sporulation.  
 
P<sub><i>cotYZ</i></sub> is a sporulation-dependent promoter out of the <i>cotYZ</i> gene cluster of <i>B. subtilis</i>, which allows for gene expression only during sporulation.  
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Information regarding the <i>cotYZ</i> operon can be found in the following databases:
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Information regarding the <i>cotYZ</i> operon can be found on the following databases:
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<html><a href="https://subtiwiki.uni-goettingen.de/">SubtiWiki</a></html>,  
 
<html><a href="https://subtiwiki.uni-goettingen.de/">SubtiWiki</a></html>,  
 
<html><a href="https://dbtbs.hgc.jp/">DBTBS</a></html>.
 
<html><a href="https://dbtbs.hgc.jp/">DBTBS</a></html>.
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P<sub><i>cotYZ</i></sub> only served for design purposes of the TU Dresden iGEM 2024 Team and was required for the construction of composite parts (see <html><a href="https://2024.igem.wiki/tu-dresden/contribution">Contribution</a></html> page).  
 
P<sub><i>cotYZ</i></sub> only served for design purposes of the TU Dresden iGEM 2024 Team and was required for the construction of composite parts (see <html><a href="https://2024.igem.wiki/tu-dresden/contribution">Contribution</a></html> page).  
  
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<b>Biosafety level:</b> S1
  
 
<b>Target organism:</b> <i>Bacillus subtilis</i>
 
<b>Target organism:</b> <i>Bacillus subtilis</i>
  
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<b>Main purpose of use:</b>: Gene expression and production of fusion proteins (especially for spore surface display)
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<b>Main purpose of use:</b> Sporulation-dependent gene expression in the host <i>B. subtilis</i>
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<b> Potential application:</b> Spore surface display
  
  

Latest revision as of 23:57, 1 October 2024


PcotYZ

This part contains the regulatory sequence of PcotYZ, which is a promoter of Bacillus subtilis. PcotYZ is a sporulation-dependent promoter out of the cotYZ gene cluster of B. subtilis, which allows for gene expression only during sporulation. Information regarding the cotYZ operon can be found in the following databases: SubtiWiki, DBTBS.

In previous studies, this promoter has so far provided the highest activity for spore surface display (Bartels et al. 2018, unpublished data of Elif Öztel).


PcotYZ only served for design purposes of the TU Dresden iGEM 2024 Team and was required for the construction of composite parts (see Contribution page).


Biosafety level: S1

Target organism: Bacillus subtilis

Main purpose of use: Sporulation-dependent gene expression in the host B. subtilis

Potential application: Spore surface display


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Bartels J., López Castellanos S., Radeck J., Mascher T. (2018): Sporobeads: The utilization of the Bacillus subtilis endospore crust as a protein display platform. ACS synthetic biology 7(2), 452-461. https://doi.org/10.1021/acssynbio.7b00285

Öztel, E. & Mascher T. (2024): unpublished data.