Difference between revisions of "Part:BBa K5207004"
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It belongs to the CYP76AH subfamily of cytochrome P450 oxygenases. It is a hybrid catalytic P450 that catalyzes the hydroxylation of ferredoxin at the C11 position and the carbonylation of tanshinone at the C7 position, generating 11-hydroxyferredoxin, lupulin, and 11-hydroxylupulin.The carbonylation function at the C7 position allows for the extension of the tanshinone biosynthesis pathway into an additional branched chain, resulting in the formation of a complex catalytic network. We inserted it into pYTK001 by method above. | It belongs to the CYP76AH subfamily of cytochrome P450 oxygenases. It is a hybrid catalytic P450 that catalyzes the hydroxylation of ferredoxin at the C11 position and the carbonylation of tanshinone at the C7 position, generating 11-hydroxyferredoxin, lupulin, and 11-hydroxylupulin.The carbonylation function at the C7 position allows for the extension of the tanshinone biosynthesis pathway into an additional branched chain, resulting in the formation of a complex catalytic network. We inserted it into pYTK001 by method above. | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K5207004 SequenceAndFeatures</partinfo> | <partinfo>BBa_K5207004 SequenceAndFeatures</partinfo> | ||
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===Functional Parameters=== | ===Functional Parameters=== | ||
<partinfo>BBa_K5207004 parameters</partinfo> | <partinfo>BBa_K5207004 parameters</partinfo> | ||
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| + | <!-- Add more about the biology of this part here--> | ||
| + | ===Usage and Biology=== | ||
| + | <html> | ||
| + | <body> | ||
| + | <figure> | ||
| + | <div class = "center"> | ||
| + | <center><img src = "https://static.igem.wiki/teams/5207/parts/1.jpg" style = "width:600px"></center> | ||
| + | </div> | ||
| + | <figcaption><center>Figure 1. Validation plot of target gene gel electrophoresis</center></figcaption> | ||
| + | </figure> | ||
| + | </body> | ||
| + | </html> | ||
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Latest revision as of 07:49, 30 September 2024
CYP76AH3
It belongs to the CYP76AH subfamily of cytochrome P450 oxygenases. It is a hybrid catalytic P450 that catalyzes the hydroxylation of ferredoxin at the C11 position and the carbonylation of tanshinone at the C7 position, generating 11-hydroxyferredoxin, lupulin, and 11-hydroxylupulin.The carbonylation function at the C7 position allows for the extension of the tanshinone biosynthesis pathway into an additional branched chain, resulting in the formation of a complex catalytic network. We inserted it into pYTK001 by method above.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
