Difference between revisions of "Part:BBa K5241011"
 
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   <td>Ionizing radiation time.</td>
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   <td><span style="padding:8px 10px;">Ionizing radiation time.</span></td>
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   <td>Control group.
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   <td><span style="padding:8px 10px;">Control group.
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（ADE2-gal1-egfp-CYC1+）</td>
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（ADE2-gal1-egfp-CYC1+）</span></td>
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   <td>Experimental group.
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   <td><span style="padding:8px 10px;">Experimental group.
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（ADE2-gal1-egfp-CYC1+）</td>
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（ADE2-gal1-egfp-CYC1+）</span></td>
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   <td>Viability of the microbial strain.</td>
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   <td><span style="padding:8px 10px;">Viability of the microbial strain.</span></td>
 
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Latest revision as of 05:42, 30 September 2024


ADE2-gal1-egfp-CYC1

Short Description:The ADE2 gene is combined with the yeast gal1 promoter, the enhanced green fluorescent protein gene egfp, and the CYC1 terminator.


genetic map：


Source:Brewing yeast

Description:

We used ADE2-gal1-egfp-CYC1 as the control group for ionizing radiation experiments, and Tyrosinase (BM) as the experimental group, to verify the radioprotective effect of melanin by comparison.

Result:

We conducted ionizing radiation experiments on ADE2-gal1-egfp-CYC1, generating a lethality curve as a control group.


Ionizing radiation time. Control group. （ADE2-gal1-egfp-CYC1+） Experimental group. （ADE2-gal1-egfp-CYC1+） Viability of the microbial strain.
1h 48.91%
4h 28.54%
8h 5.92%
12h 0

In the next phase, we will expose tyrosinase (BM) to ionizing radiation and compare the differences between the two to assess the radiation-protective effect of melanin.

[1] Veronica J Heintz, Ling Wang, Douglas J LaCount, NanoLuc luciferase as a quantitative yeast two-hybrid reporter, FEMS Yeast Research, 10.1093/femsyr/foab069, 21, 8, (2021). View.

[2] David Cluet, Ikram Amri, Blandine Vergier, Jérémie Léault, Astrid Audibert, Clémence Grosjean, Dylan Calabrési, Martin Spichty, A Quantitative Tri-fluorescent Yeast Two-hybrid System: From Flow Cytometry to In cellula Affinities, Molecular & Cellular Proteomics, 10.1074/mcp.TIR119.001692, 19, 4, (701-715), (2020).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 4
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 201
  • 1000
    COMPATIBLE WITH RFC[1000]