Difference between revisions of "Part:BBa K5439006:Design"

 
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
NcoI and XhoI restriction sites were added at the 5' and 3' ends. There are no scars between genes.  
+
NcoI and XhoI restriction sites were added at the 5' and 3' ends. There are no scars between genes due to the use of Gibson Assembly.  
  
  
Line 13: Line 13:
 
===Source===
 
===Source===
  
−
BBa_K1159302, BBa_K5439005, BBa_K1907000
+
The original sequence was reported by Murdoch, R. W., & Hay, A. G. (2013). The sequence for BBa_K1159302 was reported by Poëa-Guyon et al. (2013). The sequence for BBa_K1907000 was reported by Nagai et al. (2002).
  
 
===References===
 
===References===
 +
[1]. Murdoch, R. W., & Hay, A. G. (2013). Genetic and chemical characterization of ibuprofen degradation by Sphingomonas Ibu-2. Microbiology (Reading, England), 159(Pt 3), 621–632. https://doi.org/10.1099/mic.0.062273-0
 +
 +
[2]. Poëa-Guyon, S., Pasquier, H., Mérola, F., Morel, N., & Erard, M. (2013). The enhanced cyan fluorescent protein: a sensitive pH sensor for fluorescence lifetime imaging. Analytical and bioanalytical chemistry, 405(12), 3983–3987. https://doi.org/10.1007/s00216-013-6860-y
 +
 +
[3]. Stassi, D., Donadio, S., Staver, M. J., & Katz, L. (1993). Identification of a Saccharopolyspora erythraea gene required for the final hydroxylation step in erythromycin biosynthesis. Journal of bacteriology, 175(1), 182–189. https://doi.org/10.1128/jb.175.1.182-189.1993

Latest revision as of 02:30, 2 October 2024


FRET-based system for the detection of ibuprofen


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 2306
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1177
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2174
    Illegal BsaI.rc site found at 2959


Design Notes

NcoI and XhoI restriction sites were added at the 5' and 3' ends. There are no scars between genes due to the use of Gibson Assembly.


Source

The original sequence was reported by Murdoch, R. W., & Hay, A. G. (2013). The sequence for BBa_K1159302 was reported by Poëa-Guyon et al. (2013). The sequence for BBa_K1907000 was reported by Nagai et al. (2002).

References

[1]. Murdoch, R. W., & Hay, A. G. (2013). Genetic and chemical characterization of ibuprofen degradation by Sphingomonas Ibu-2. Microbiology (Reading, England), 159(Pt 3), 621–632. https://doi.org/10.1099/mic.0.062273-0

[2]. Poëa-Guyon, S., Pasquier, H., Mérola, F., Morel, N., & Erard, M. (2013). The enhanced cyan fluorescent protein: a sensitive pH sensor for fluorescence lifetime imaging. Analytical and bioanalytical chemistry, 405(12), 3983–3987. https://doi.org/10.1007/s00216-013-6860-y

[3]. Stassi, D., Donadio, S., Staver, M. J., & Katz, L. (1993). Identification of a Saccharopolyspora erythraea gene required for the final hydroxylation step in erythromycin biosynthesis. Journal of bacteriology, 175(1), 182–189. https://doi.org/10.1128/jb.175.1.182-189.1993