Difference between revisions of "Part:BBa K208011:Design"

(→‎Design Notes)
 
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===Design Notes===
 
===Design Notes===
−
In pSB1AK3.
+
This is a variation of a Lac promoter with an attached ribosome binding site. The RBS was attached to the promoter and made into a BioBrick to simplify the composite part construction process as cutting and ligating a small sequence, like a ribosome binding site, can be difficult.
  
 
===Source===
 
===Source===
  
−
BBa_I3601
+
This part was designed by the USU iGEM 2009 team and synthesized by DNA 2.0. The part was cut and ligated into a BioBrick plasmid, pSB1AK3 and sequenced.
  
 
===References===
 
===References===
 +
Please consult the original pages for these parts for more details on their functionality.

Latest revision as of 01:03, 23 October 2009

Lac Reg. Lambda Hybrid (BBa_R0011) and RBS (BBa_B0034)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This is a variation of a Lac promoter with an attached ribosome binding site. The RBS was attached to the promoter and made into a BioBrick to simplify the composite part construction process as cutting and ligating a small sequence, like a ribosome binding site, can be difficult.

Source

This part was designed by the USU iGEM 2009 team and synthesized by DNA 2.0. The part was cut and ligated into a BioBrick plasmid, pSB1AK3 and sequenced.

References

Please consult the original pages for these parts for more details on their functionality.