Difference between revisions of "Part:BBa K5324001:Design"

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===Design Notes===
 
===Design Notes===
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This sequence was codon optimized for expression in E.coli. Bsal and Bsml restriction cut sites removed with silent mutations.
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This sequence was codon optimized for expression in E.coli. Restriction cut sites were avoided for BsaI, BsmI, and SapI.
  
 
===Source===
 
===Source===
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===References===
 
===References===
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Bennett, R. J. (2015, April 15). Destructin-1 is a collagen-degrading endopeptidase ... Destructin-1 is a collagen-degrading endopeptidase secreted by Pseudogymnoascus destructans, the causative agent of white-nose syndrome. https://www.pnas.org/doi/full/10.1073/pnas.1507082112  
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1 - Bennett, R. J. (2015, April 15). Destructin-1 is a collagen-degrading endopeptidase ... Destructin-1 is a collagen-degrading endopeptidase secreted by Pseudogymnoascus destructans, the causative agent of white-nose syndrome. https://www.pnas.org/doi/full/10.1073/pnas.1507082112 <br>
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U.S. National Library of Medicine. (n.d.). RecName: Full=subtilisin-like protease 2; AltName: Full=destructin-1;. National Center for Biotechnology Information. https://www.ncbi.nlm.nih.gov/protein/L8FSM5.1
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2 - U.S. National Library of Medicine. (n.d.). RecName: Full=subtilisin-like protease 2; AltName: Full=destructin-1;. National Center for Biotechnology Information. https://www.ncbi.nlm.nih.gov/protein/L8FSM5.1

Latest revision as of 22:07, 29 September 2024


Destructin-1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 55
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 832
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence was codon optimized for expression in E.coli. Restriction cut sites were avoided for BsaI, BsmI, and SapI.

Source

Genomic sequence obtained from GeneBank. Originated from P. destructans.

References

1 - Bennett, R. J. (2015, April 15). Destructin-1 is a collagen-degrading endopeptidase ... Destructin-1 is a collagen-degrading endopeptidase secreted by Pseudogymnoascus destructans, the causative agent of white-nose syndrome. https://www.pnas.org/doi/full/10.1073/pnas.1507082112
2 - U.S. National Library of Medicine. (n.d.). RecName: Full=subtilisin-like protease 2; AltName: Full=destructin-1;. National Center for Biotechnology Information. https://www.ncbi.nlm.nih.gov/protein/L8FSM5.1