Difference between revisions of "Part:BBa K5499005"

 
 
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The signal peptide of LAMP2A is a short amino acid sequence (typically around 15-30 residues) that serves as a molecular "address" that directs the protein to the secretory pathway. Here are key roles the LAMP2A signal peptide plays:
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The signal peptide of LAMP2A is a short amino acid sequence (typically around 15-30 residues) that serves as a molecular "address" that directs the protein to the secretory pathway.  
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Translocation to the ER: The signal peptide facilitates the translocation of the LAMP2A precursor protein into the lumen of the ER. This is essential for proper folding and modification (e.g., glycosylation).
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Membrane Integration: After processing in the ER, the signal peptide is cleaved off, and the mature LAMP2A protein is integrated into the lysosomal membrane, where it can perform its functions.
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If you are interested in exosome-based drug delivery, incorporating the LAMP2A signal peptide can help direct proteins into exosomes. This can increase the efficiency of exosome production and enhance the therapeutic potential of exosome-mediated delivery systems.
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===Usage and Biology===
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===Profile===
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Name: LAMP2A Signal Peptide
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Base Pairs: 84 bp
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Origins: Derived from the lysosomal-associated membrane protein 2 (LAMP2) gene, commonly found in mammals.
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Properties: It selectively recognizes cytosolic proteins containing specific amino acid sequences, such as the KFERQ motif, which are essential for their translocation into exosomes.
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===Usage and Biology===
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The LAMP2A signal peptide is commonly utilized in synthetic biology and genetic engineering to direct protein localization to exosomes. By attaching this signal peptide to the N-terminus of recombinant proteins, researchers can ensure the correct localization of intracellular proteins on the exosomal membrane, which is crucial for the functionality of exosomes and proteins.
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This precise targeting is particularly important in therapeutic applications, where exosomes serve as natural vehicles for drug delivery and intercellular communication.

Latest revision as of 21:36, 1 October 2024


LAMP2A signal peptide

The signal peptide of LAMP2A is a short amino acid sequence (typically around 15-30 residues) that serves as a molecular "address" that directs the protein to the secretory pathway.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Profile

Name: LAMP2A Signal Peptide

Base Pairs: 84 bp

Origins: Derived from the lysosomal-associated membrane protein 2 (LAMP2) gene, commonly found in mammals.

Properties: It selectively recognizes cytosolic proteins containing specific amino acid sequences, such as the KFERQ motif, which are essential for their translocation into exosomes.

Usage and Biology

The LAMP2A signal peptide is commonly utilized in synthetic biology and genetic engineering to direct protein localization to exosomes. By attaching this signal peptide to the N-terminus of recombinant proteins, researchers can ensure the correct localization of intracellular proteins on the exosomal membrane, which is crucial for the functionality of exosomes and proteins.

This precise targeting is particularly important in therapeutic applications, where exosomes serve as natural vehicles for drug delivery and intercellular communication.