Difference between revisions of "Part:BBa K5117000"

Latest revision as of 23:35, 1 October 2024

BsRBS

This part consists of the ribosome binding site of Bacillus subtilis (already described by Vellanoweth & Rabinowitz 1992) followed by a 7 bp spacer.

BsRBS only served for design purposes of the TU Dresden iGEM 2024 Team and was required for the construction of composite parts (see Contribution page).

Biosafety level: S1

Target organism: Bacillus subtilis

Main purpose of use: Gene expression and protein production using the host B. subtilis

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

References

Vellanoweth R. L. & Rabinowitz J. C. (1992): The influence of ribosome‐binding‐site elements on translational efficiency in Bacillus subtilis and Escherichia coli in vivo. Molecular microbiology 6(9), 1105-1114. https://doi.org/10.1111/j.1365-2958.1992.tb01548.x

@@ Line 5: / Line 5: @@
 This part consists of the ribosome binding site of <i>Bacillus subtilis</i> (already described by Vellanoweth & Rabinowitz 1992) followed by a 7 bp spacer.
-BsRBS only served for design purposes of the TU Dresden iGEM 2024 Team. This part was neither built nor characterized in the laboratory.
+BsRBS only served for design purposes of the TU Dresden iGEM 2024 Team and was required for the construction of composite parts (see <html><a href="https://2024.igem.wiki/tu-dresden/contribution">Contribution</a></html> page).
+<b>Biosafety level:</b> S1
 <b>Target organism:</b> <i>Bacillus subtilis</i>
-<b>Main purpose of use:</b> Gene expression and protein production using the host <i>Bacillus subtilis</i>
+<b>Main purpose of use:</b> Gene expression and protein production using the host <i>B. subtilis</i>