Difference between revisions of "Part:BBa K5108003"
 
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<ol style="color: black; padding: auto -1rem; margin= 0"> <b>Contents</b>
 
<ol style="color: black; padding: auto -1rem; margin= 0"> <b>Contents</b>
 
     <li style="color: blue;">Usage and Biology</li>
 
     <li style="color: blue;">Usage and Biology</li>
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<li style="color: blue;">Characterization and Measurements</li>
 
     <li style="color: blue;">References</li>
 
     <li style="color: blue;">References</li>
 
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<p><a href="https://www.uniprot.org/uniprotkb/P38488/entry" target="blank">Creatinase</a> (CreA) is an enzyme that catalyzes the degradation of creatine into sarcosine in <i>Pseudomonas putida</i>.  
 
<p><a href="https://www.uniprot.org/uniprotkb/P38488/entry" target="blank">Creatinase</a> (CreA) is an enzyme that catalyzes the degradation of creatine into sarcosine in <i>Pseudomonas putida</i>.  
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In the context of our project, the gene encoding this enzyme (<i>creA</i>) was synthesized in an operon together with the gene for creatine degradation. You can find more information about construct and our results in <a href="https://parts.igem.org/Part:BBa_K5108009" target="blank">BBa_K5108009</a>. Figure 1 displays the intermediate step of construction.
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In the context of our project, the gene encoding this enzyme (<i>creA</i>) was synthesized in an operon together with the gene for creatine degradation. You can find more information about construct in <a href="https://parts.igem.org/Part:BBa_K5108009" target="blank">BBa_K5108009.</a></p>
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            <img class="d-block"
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            src="https://static.igem.wiki/teams/5108/lea/crea-part.png"><br><br>
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            <figcaption class="normal"><span class="titre-image"><b>Figure 1: Design of the expression plasmid harboring the <i>creA</i> gene.</b></span></figcaption>
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        </figure>
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<h2 style="color: blue;"><b>Characterization and Measurements</b></h2>
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<p>SDS-PAGE, growth analysis and consumption analysis of creatinine by NMR spectroscopy were performed. The results can be found in <a href="https://parts.igem.org/Part:BBa_K5108009" target="blank">BBa_K5108009</a> and in <a href="https://2024.igem.wiki/toulouse-insa-ups/results">iGEM ToulouseINSA-UPS 2024.</a></p>
 
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<h2 style="color: blue;"><b>References</b></h2>
 
<h2 style="color: blue;"><b>References</b></h2>
  

Latest revision as of 14:17, 1 October 2024

Creatinase from Pseudomonas putida

Creatinase from Pseudomonas putida

    Contents
  1. Usage and Biology
  2. Characterization and Measurements
  3. References



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 225
    Illegal BsaI site found at 1012

Usage and Biology

Creatinase (CreA) is an enzyme that catalyzes the degradation of creatine into sarcosine in Pseudomonas putida. In the context of our project, the gene encoding this enzyme (creA) was synthesized in an operon together with the gene for creatine degradation. You can find more information about construct in BBa_K5108009.


Characterization and Measurements

SDS-PAGE, growth analysis and consumption analysis of creatinine by NMR spectroscopy were performed. The results can be found in BBa_K5108009 and in iGEM ToulouseINSA-UPS 2024.


References

  • UniProt. (s. d.-b). https://www.uniprot.org/uniprotkb/P38488/entry
  • Cloning, Expression and Purification of Pseudomonas putida ATCC12633 Creatinase. (2017, 1 décembre). PubMed. https://pubmed.ncbi.nlm.nih.gov/29090065/
    •