Difference between revisions of "Part:BBa K243032:Design"
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===Design Notes===
 
===Design Notes===
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The combination of the parts [https://parts.igem.org/Part:BBa_243000 Fok_a] and [https://parts.igem.org/Part:BBa_I757008 mVenus-YFP] was done to gain information about protein expression. The mVenusYFP is a proven Biobrick of the [http://parts.mit.edu/igem07/index.php/Freiburg team Freiburg07]. Thus, Venus fusion proteins give an easy to visualize readout of protein expression and are also good detectable by western blotting.
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The combination of the parts [https://parts.igem.org/Part:BBa_243000 Fok_a] and [https://parts.igem.org/Part:BBa_I757008 mVenus-YFP] was done to gain information about protein expression. The mVenusYFP is a proven Biobrick of the [http://2007.igem.org/Freiburg team Freiburg07]. Thus, Venus fusion proteins give an easy to visualize readout of protein expression and are also good detectable by western blotting.
  
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Designed according the [https://parts.igem.org/Assembly_standard_25 RFC 25].
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Designed according to the [https://parts.igem.org/Assembly_standard_25 RFC 25].
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[https://static.igem.org/mediawiki/parts/7/79/Freibur09_Venus.txt Commented GenBank file]
  
 
===Source===
 
===Source===

Latest revision as of 01:10, 22 October 2009

Fok_a-Venus


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 487


Design Notes

The combination of the parts Fok_a and mVenus-YFP was done to gain information about protein expression. The mVenusYFP is a proven Biobrick of the [http://2007.igem.org/Freiburg team Freiburg07]. Thus, Venus fusion proteins give an easy to visualize readout of protein expression and are also good detectable by western blotting.

Designed according to the RFC 25. Commented GenBank file

Source

Combined the parts by serial cloning steps.

References