Difference between revisions of "Part:BBa K5477008"
 
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<partinfo>BBa_K5477008 short</partinfo>
 
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<i>XRE</i> is Xenobiotic Response Element found in eukaryotic cell upstream of inducible target genes for the transcription factor aryl hydrocarbon receptor (<i>AhR</i>) [https://parts.igem.org/Part:BBa_K3793004| BBa_K3793004]  which is responsible for signal transduction of pollutants (1) (2). XRE was used in our reporter module to drive the expression of <i>NanoLuc</i> under the presence of PAHs or PCB [https://parts.igem.org/Part:BBa_K5477030| BBa_K5477030].  This reporter module is combined with the AhR receptor modules and therefore comprises our biosensor systems to detect PAHs and PCBs [https://parts.igem.org/Part:BBa_K5477041| BBa_K5477041] and [https://parts.igem.org/Part:BBa_K5477042| BBa_K5477042] .
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Xenobiotic Response Element (<i>XRE</i>) or dioxin response element (<i>DRE</i>) is in eukaryotic cell upstream of inducible target genes for the ligand-activated transcription factor aryl hydrocarbon receptor (<i>AhR</i>) [https://parts.igem.org/Part:BBa_K3793004 BBa_K3793004]  which is responsible for signal transduction of pollutants (1) (2).  
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XRE was used in the reporter module to drive the expression of <i>NanoLuc</i> under the presence of PAHs or PCB [https://parts.igem.org/Part:BBa_K5477030 BBa_K5477030].  This reporter module was combined with the AhR receptor modules and therefore comprises the following biosensor devices to detect PAHs and PCBs (Aroclor 1260) [https://parts.igem.org/Part:BBa_K5477041 BBa_K5477041] and [https://parts.igem.org/Part:BBa_K5477042 BBa_K5477042] .
  
  

Latest revision as of 20:57, 1 October 2024


XRE - Xenobiotic Response Element

Xenobiotic Response Element (XRE) or dioxin response element (DRE) is in eukaryotic cell upstream of inducible target genes for the ligand-activated transcription factor aryl hydrocarbon receptor (AhR) BBa_K3793004 which is responsible for signal transduction of pollutants (1) (2).

XRE was used in the reporter module to drive the expression of NanoLuc under the presence of PAHs or PCB BBa_K5477030. This reporter module was combined with the AhR receptor modules and therefore comprises the following biosensor devices to detect PAHs and PCBs (Aroclor 1260) BBa_K5477041 and BBa_K5477042 .


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 59
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 59
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 59
    Illegal BamHI site found at 3
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 59
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 59
  • 1000
    COMPATIBLE WITH RFC[1000]


References

1. Huang G, Elferink CJ. A novel nonconsensus xenobiotic response element capable of mediating aryl hydrocarbon receptor-dependent gene expression. Mol Pharmacol. 2012 Mar;81(3):338-47. doi: 10.1124/mol.111.075952. Epub 2011 Nov 23. PMID: 22113079; PMCID: PMC3286297.

2. Kuramoto, Nobuyuki & Baba, Katsuhiro & Gion, Keiko & Sugiyama, Chie & Taniura, Hideo & Yoneda, Yukio. (2003). Xenobiotic response element binding enriched in both nuclear and microsomal fractions of rat cerebellum. Journal of neurochemistry. 85. 264-73. 10.1046/j.1471-4159.2003.01679.x.