Difference between revisions of "Part:BBa K211002:Design"
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Designed with PCR. | Designed with PCR. | ||
[[Image:primer design.jpg]] | [[Image:primer design.jpg]] | ||
| − | Primer Sequence | + | Primer Sequence |
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P1 RI7-N F ATATCTGAATTCGCCACCATGGAACGAAGGAACCAC | P1 RI7-N F ATATCTGAATTCGCCACCATGGAACGAAGGAACCAC | ||
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| − | BioBrick F ATATCT TCTAGATGGAACGAAGGAACCAC | + | BioBrick F ATATCT TCTAGATGGAACGAAGGAACCAC |
| + | |||
BioBrick R GTATACACTAGTA TTA TTA ACCAATTTTGCTGCC | BioBrick R GTATACACTAGTA TTA TTA ACCAATTTTGCTGCC | ||
Latest revision as of 22:09, 21 October 2009
RI7-odr10 chimeric GPCR
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 761
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Designed with PCR.
Primer Sequence
P1 RI7-N F ATATCTGAATTCGCCACCATGGAACGAAGGAACCAC
P2 RI7-C R GTATACGCGGCCGCACCAATTTTGCTGCCTTTG
P3 RI7-N R GAGAAGAA ATACATGGGTTTGTGGAG
P4 Odr-10 F CCATGTAT TTCTTCTCAGTATTCTCG
P5 Odr-10 R GCAGTA AATGATCAGAATCAGAATGAG
P6 RI7-C F GATCATT TACTGCTTGCGCAACC
BioBrick F ATATCT TCTAGATGGAACGAAGGAACCAC
BioBrick R GTATACACTAGTA TTA TTA ACCAATTTTGCTGCC
We would like to thank Professor Piali Sengupta, Department of Biology, Brandeis University for the kind gift of the odr-10 cDNA, and Professor Stuart Firestein, Department of Biological Sciences, Columbia University for the kind gift of the rat I7 olfactory receptor cDNA.
Source
Caenorhabditis elegans olfactory receptor Odr-10 (GenBank: U49449.1)
Rat olfactory protein, clone I7 (GenBank: M64386.1)
References
Radhika, V. et al . 2007. Chemical sensing of DNT by engineered olfactory yeast strain. Nat. Chem. Biol. 3 : 325–330