Difference between revisions of "Part:BBa I731012:Experience"

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<html><a href="https://igem.org/Image:BBa_I731012-check.png" target="_blank"> <img src="https://igem.org/Image:BBa_I731012-check.png" width="400" height="300"></a></html>
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<!-- DON'T DELETE --><partinfo>BBa_I731012 EndReviews</partinfo>
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The Chiba iGEM team tested this part in several conditions.
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*Without LuxI (or synthetic OHHL), RFP levels increase.
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Experiment : checking  BBa_I731012
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#Picked and cultured the following stab and glycerol stocks in 2mL of LB:
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##LB-Amp, BBa_I731012, (stab, received from iGEM)
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##LB-Amp, BBa_K084007(plac+rbs+LasI(no LVA)), (JW1908)
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##LB-Amp, BBa_K084012(plac+rbs+LuxI(no LVA)), (JW1908)
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#Pre-Cultured at 37°C for 12h.
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#Added 6.25% each of the pre-cultures to new LB medium.
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##LB-Amp, BBa_T9002, BBa_K084012(plac+rbs+LuxI(no LVA)), BBa_K084007(plac+rbs+LasI(no LVA))
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#Cultured at 37°C for 12h
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#Mixed the sender cells BBa_K08400 and 7BBa_K084012 both with BBa_I1731012 at a 1:1 ratio.
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##BBa_I1731012 alone.
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##BBa_K084007:BBa_BBa_I1731012=1:1
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##BBa_K084012:BBa_I1731012=1:1
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#Culture 12h and observe results(follow picture).
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<html><a href="https://static.igem.org/mediawiki/igem.org/0/0e/BBa_I731012-check.png" target="_blank"> <img src="https://static.igem.org/mediawiki/igem.org/0/0e/BBa_I731012-check.png" width="400" height="300"></a></html>
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*Funakoshi, UVGL-58 : long wave(365 nm)

Latest revision as of 03:55, 22 October 2009

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_I731012

User Reviews

UNIQ11b18e8729c93ed9-partinfo-00000000-QINU UNIQ11b18e8729c93ed9-partinfo-00000001-QINU

The Chiba iGEM team tested this part in several conditions.

  • Without LuxI (or synthetic OHHL), RFP levels increase.

Experiment : checking BBa_I731012

  1. Picked and cultured the following stab and glycerol stocks in 2mL of LB:
    1. LB-Amp, BBa_I731012, (stab, received from iGEM)
    2. LB-Amp, BBa_K084007(plac+rbs+LasI(no LVA)), (JW1908)
    3. LB-Amp, BBa_K084012(plac+rbs+LuxI(no LVA)), (JW1908)
  2. Pre-Cultured at 37°C for 12h.
  3. Added 6.25% each of the pre-cultures to new LB medium.
    1. LB-Amp, BBa_T9002, BBa_K084012(plac+rbs+LuxI(no LVA)), BBa_K084007(plac+rbs+LasI(no LVA))
  4. Cultured at 37°C for 12h
  5. Mixed the sender cells BBa_K08400 and 7BBa_K084012 both with BBa_I1731012 at a 1:1 ratio.
    1. BBa_I1731012 alone.
    2. BBa_K084007:BBa_BBa_I1731012=1:1
    3. BBa_K084012:BBa_I1731012=1:1
  6. Culture 12h and observe results(follow picture).

  • Funakoshi, UVGL-58 : long wave(365 nm)