Difference between revisions of "Part:BBa K5108003"
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<partinfo>BBa_K5108003 short</partinfo> | <partinfo>BBa_K5108003 short</partinfo> | ||
| − | + | Creatinase from <i>Pseudomonas putida</i> | |
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<ol style="color: black; padding: auto -1rem; margin= 0"> <b>Contents</b> | <ol style="color: black; padding: auto -1rem; margin= 0"> <b>Contents</b> | ||
<li style="color: blue;">Usage and Biology</li> | <li style="color: blue;">Usage and Biology</li> | ||
| + | <li style="color: blue;">Characterization and Measurements</li> | ||
<li style="color: blue;">References</li> | <li style="color: blue;">References</li> | ||
</ol> | </ol> | ||
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<h2 style="color: blue;"> <b>Usage and Biology</b></h2> | <h2 style="color: blue;"> <b>Usage and Biology</b></h2> | ||
| − | <p><a href="https://www.uniprot.org/uniprotkb/P38488/entry" target="blank"> | + | <p><a href="https://www.uniprot.org/uniprotkb/P38488/entry" target="blank">Creatinase</a> (CreA) is an enzyme that catalyzes the degradation of creatine into sarcosine in <i>Pseudomonas putida</i>. |
| − | In the context of our project, the gene encoding this enzyme (<i>creA</i>) was synthesized in an operon with the gene for creatine degradation. You can find more information about | + | In the context of our project, the gene encoding this enzyme (<i>creA</i>) was synthesized in an operon together with the gene for creatine degradation. You can find more information about construct in <a href="https://parts.igem.org/Part:BBa_K5108009" target="blank">BBa_K5108009.</a></p> |
| − | </p> | + | <br> |
| + | <h2 style="color: blue;"><b>Characterization and Measurements</b></h2> | ||
| + | <p>SDS-PAGE, growth analysis and consumption analysis of creatinine by NMR spectroscopy were performed. The results can be found in <a href="https://parts.igem.org/Part:BBa_K5108009" target="blank">BBa_K5108009</a> and in <a href="https://2024.igem.wiki/toulouse-insa-ups/results">iGEM ToulouseINSA-UPS 2024.</a></p> | ||
| + | <br> | ||
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<h2 style="color: blue;"><b>References</b></h2> | <h2 style="color: blue;"><b>References</b></h2> | ||
| − | < | + | <il> |
<i> | <i> | ||
<li>UniProt. (s. d.-b). https://www.uniprot.org/uniprotkb/P38488/entry</li> | <li>UniProt. (s. d.-b). https://www.uniprot.org/uniprotkb/P38488/entry</li> | ||
<li>Cloning, Expression and Purification of Pseudomonas putida ATCC12633 Creatinase. (2017, 1 décembre). PubMed. https://pubmed.ncbi.nlm.nih.gov/29090065/</li> | <li>Cloning, Expression and Purification of Pseudomonas putida ATCC12633 Creatinase. (2017, 1 décembre). PubMed. https://pubmed.ncbi.nlm.nih.gov/29090065/</li> | ||
</i> | </i> | ||
| − | </ | + | </il> |
</html> | </html> | ||
Latest revision as of 14:17, 1 October 2024
Creatinase from Pseudomonas putida
Creatinase from Pseudomonas putida
- Contents
- Usage and Biology
- Characterization and Measurements
- References
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 225
Illegal BsaI site found at 1012
Usage and Biology
Creatinase (CreA) is an enzyme that catalyzes the degradation of creatine into sarcosine in Pseudomonas putida. In the context of our project, the gene encoding this enzyme (creA) was synthesized in an operon together with the gene for creatine degradation. You can find more information about construct in BBa_K5108009.
Characterization and Measurements
SDS-PAGE, growth analysis and consumption analysis of creatinine by NMR spectroscopy were performed. The results can be found in BBa_K5108009 and in iGEM ToulouseINSA-UPS 2024.