Difference between revisions of "Part:BBa K5108004"
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<partinfo>BBa_K5108004 short</partinfo> | <partinfo>BBa_K5108004 short</partinfo> | ||
| + | Creatinine amidohydrolase from <i>Pseudomonas putida</i> | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
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<ol style="color: black; padding: auto -1rem; margin= 0"> <b>Contents</b> | <ol style="color: black; padding: auto -1rem; margin= 0"> <b>Contents</b> | ||
<li style="color: blue;">Usage and Biology</li> | <li style="color: blue;">Usage and Biology</li> | ||
| + | <li style="color: blue;">Characterization and Measurements</li> | ||
<li style="color: blue;">References</li> | <li style="color: blue;">References</li> | ||
</ol> | </ol> | ||
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<h2 style="color: blue;"> <b>Usage and Biology</b></h2> | <h2 style="color: blue;"> <b>Usage and Biology</b></h2> | ||
| − | <p><a href="https://www.uniprot.org/uniprotkb/P83772/entry" target="blank"> | + | <p><a href="https://www.uniprot.org/uniprotkb/P83772/entry" target="blank">Creatinine amidohydrolase</a> (CrnA) is an enzyme that catalyzes the degradation of creatinine into creatine in <i>Pseudomonas putida</i>. |
| − | In the context of our project, the gene encoding this enzyme (<i>crnA</i>) was synthesized in an operon with the gene for creatine degradation. You can find more | + | In the context of our project, the gene encoding this enzyme (<i>crnA</i>) was synthesized in an operon together with the gene for creatine degradation. You can find more informations about construct and results in <a href="https://parts.igem.org/Part:BBa_K5108009" target="blank">BBa_K5108009.</a> </p> |
| − | </p> | + | <br> |
| + | <h2 style="color: blue;"><b>Characterization and Measurements</b></h2> | ||
| + | <p>SDS-PAGE, growth analysis and consumption analysis of creatinine by NMR spectroscopy were performed. The results can be found in <a href="https://parts.igem.org/Part:BBa_K5108009" target="blank">BBa_K5108009</a> and in <a href="https://2024.igem.wiki/toulouse-insa-ups/results">iGEM ToulouseINSA-UPS 2024.</a></p> | ||
<br> | <br> | ||
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<h2 style="color: blue;"><b>References</b></h2> | <h2 style="color: blue;"><b>References</b></h2> | ||
| − | < | + | <il> |
<i> | <i> | ||
<li>UniProt. (s. d.). https://www.uniprot.org/uniprotkb/P83772/entry</li> | <li>UniProt. (s. d.). https://www.uniprot.org/uniprotkb/P83772/entry</li> | ||
<li>Tsuru, D., Oka, I., & Yoshimoto, T. (1976c). Creatinine Decomposing Enzymes inPseudomonas putida. Agricultural And Biological Chemistry, 40(5), 1011‑1018. https://doi.org/10.1080/00021369.1976.10862151</li> | <li>Tsuru, D., Oka, I., & Yoshimoto, T. (1976c). Creatinine Decomposing Enzymes inPseudomonas putida. Agricultural And Biological Chemistry, 40(5), 1011‑1018. https://doi.org/10.1080/00021369.1976.10862151</li> | ||
</i> | </i> | ||
| − | </ | + | </il> |
</html> | </html> | ||
Latest revision as of 14:19, 1 October 2024
Creatinine amidohydrolase form Pseudomonas putida
Creatinine amidohydrolase from Pseudomonas putida
- Contents
- Usage and Biology
- Characterization and Measurements
- References
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 61
Illegal NgoMIV site found at 663 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 199
Illegal BsaI.rc site found at 547
Usage and Biology
Creatinine amidohydrolase (CrnA) is an enzyme that catalyzes the degradation of creatinine into creatine in Pseudomonas putida. In the context of our project, the gene encoding this enzyme (crnA) was synthesized in an operon together with the gene for creatine degradation. You can find more informations about construct and results in BBa_K5108009.
Characterization and Measurements
SDS-PAGE, growth analysis and consumption analysis of creatinine by NMR spectroscopy were performed. The results can be found in BBa_K5108009 and in iGEM ToulouseINSA-UPS 2024.