Difference between revisions of "Part:BBa K5108006"
(Usage and Biology)
 
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<partinfo>BBa_K5108006 short</partinfo>
 
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Ribosome Binding Site of <i>aprA</i> gene from <i>P. protogens</i>
  
 
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<h2 style="color: blue;"> <b>Usage and Biology</b></h2>
 
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<p><a href="https://www.uniprot.org/uniprotkb/P83772/entry" target="blank">CrnA</a> is an enzyme that catalyzes the degradation of creatinine into creatine in <i>Pseudomonas putida</i>.  
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<p>Ribosome Binding Site of <i>aprA</i> gene from <i>P. protogens</i> (CHAO). It was used for protein translation in <i>P. fluorescens</i> [1].
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In the context of our project, the gene encoding this enzyme (<i>crnA</i>) was synthesized in an operon with the gene for creatine degradation. You can find more information about our construct and our results in <a href="https://parts.igem.org/Part:BBa_K5108009" target="blank">BBa_K5108009</a>.  
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This RBS was synthesized in an operon with two genes and another RBS, and cloned into a plasmid. After transformation in <i>P. fluorescens</i>, our team had successful protein expression with this RBS. Check out composite part <a href="https://parts.igem.org/Part:BBa_K5108009" target="blank">BBa_K5108009</a>for more information.  
 
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<h2 style="color: blue;"><b>References</b></h2>
 
<h2 style="color: blue;"><b>References</b></h2>
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<li>UniProt. (s. d.). https://www.uniprot.org/uniprotkb/P83772/entry</li>
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<li>Blumer, C., Heeb, S., Pessi, G., & Haas, D. (1999). Global GacA-steered control of cyanide and exoprotease production in Pseudomonas fluorescens involves specific ribosome binding sites. Proceedings Of The National Academy Of Sciences, 96(24), 14073‑14078. https://doi.org/10.1073/pnas.96.24.14073</li>
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<li>Tsuru, D., Oka, I., & Yoshimoto, T. (1976c). Creatinine Decomposing Enzymes inPseudomonas putida. Agricultural And Biological Chemistry, 40(5), 1011‑1018. https://doi.org/10.1080/00021369.1976.10862151</li>
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Latest revision as of 06:21, 1 October 2024

aprA RBS from Pseudomonas protegens (CHA0)

Ribosome Binding Site of aprA gene from P. protogens


    Contents
  1. Usage and Biology
  2. References



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and Biology

Ribosome Binding Site of aprA gene from P. protogens (CHAO). It was used for protein translation in P. fluorescens [1]. This RBS was synthesized in an operon with two genes and another RBS, and cloned into a plasmid. After transformation in P. fluorescens, our team had successful protein expression with this RBS. Check out composite part BBa_K5108009for more information.


References

  1. Blumer, C., Heeb, S., Pessi, G., & Haas, D. (1999). Global GacA-steered control of cyanide and exoprotease production in Pseudomonas fluorescens involves specific ribosome binding sites. Proceedings Of The National Academy Of Sciences, 96(24), 14073‑14078. https://doi.org/10.1073/pnas.96.24.14073
    2.