Difference between revisions of "Part:BBa K5271006"

Latest revision as of 09:36, 29 September 2024

Linker

A 3x GSSG linker connecting HER2 and EGFR nanobody domains.

Usage and Biology

The GS linker is a 12 amino acid long sequence containing 3x GSSG tandems connecting HER2 and EGFR nanobody. This basic part ensure a spatial separation between the two nanobody domains to achieve antigen binding or improve protein folding. The DNA sequence has performed codon optimization for expression in E.Coli.

Reference

Chen, X., Zaro, J. L., & Shen, W. C. (2013). Fusion protein linkers: property, design and functionality. Advanced drug delivery reviews, 65(10), 1357-1369.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 A 3x GSSG linker connecting HER2 and EGFR nanobody domains.
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 ===Usage and Biology===
-The GS linker is a 12 amino acid long sequence containing 3x GSSG tandems connecting HER2 and EGFR nanobody. This basic part ensure a spatial separation between the two nanobody domains to achieve antigen binding or improve protein folding
+The GS linker is a 12 amino acid long sequence containing 3x GSSG tandems connecting HER2 and EGFR nanobody. This basic part ensure a spatial separation between the two nanobody domains to achieve antigen binding or improve protein folding. The DNA sequence has performed codon optimization for expression in ''E.Coli''.
 ===Reference===
-*Chen, X.; Zaro, J. L.; Shen, W.-C. Fusion Protein Linkers: Property, Design and Functionality. Advanced Drug Delivery Reviews 2013, 65 (10), 1357–1369. https://doi.org/10.1016/j.addr.2012.09.039.
+*Chen, X., Zaro, J. L., & Shen, W. C. (2013). Fusion protein linkers: property, design and functionality. Advanced drug delivery reviews, 65(10), 1357-1369.
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