Difference between revisions of "Part:BBa K180010"
 
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Composite part, consisting of BBa_K180011 and BBa_K180012, but with BBa_K180011 reading along reverse strand, and BBa_K180012 reading along the forward strand.
 
Composite part, consisting of BBa_K180011 and BBa_K180012, but with BBa_K180011 reading along reverse strand, and BBa_K180012 reading along the forward strand.
  
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BBa_K180011 reading along reverse strand, and BBa_K180012 reading along the forward strand to ensure the constitutive promoter BBa_J23119 (present in BBa_K180011) has no effect over mCherry or LasI expression in BBa_K180012.
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BBa_K180011 reads along reverse strand, and BBa_K180012 reads along the forward strand to ensure the constitutive promoter BBa_J23119 (present in BBa_K180011) has no effect over mCherry or LasI expression in BBa_K180012.
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mCherry and LasI are generated under regulation by tac pR, whilst RhlR is produced constitutively.
  
 
Forms the 'rock' primary plasmid in Team:Southampton's rock-paper-scissors project design.
 
Forms the 'rock' primary plasmid in Team:Southampton's rock-paper-scissors project design.

Latest revision as of 21:24, 21 October 2009

Rock-paper-scissors - Rock primary plasmid

Composite part, consisting of BBa_K180011 and BBa_K180012, but with BBa_K180011 reading along reverse strand, and BBa_K180012 reading along the forward strand.

BBa_K180011 reads along reverse strand, and BBa_K180012 reads along the forward strand to ensure the constitutive promoter BBa_J23119 (present in BBa_K180011) has no effect over mCherry or LasI expression in BBa_K180012.

mCherry and LasI are generated under regulation by tac pR, whilst RhlR is produced constitutively.

Forms the 'rock' primary plasmid in Team:Southampton's rock-paper-scissors project design.


Usage and Biology

This part has been specified as a basic BioBrick, due to some of the component parts reading in reverse (unable to specify using the component BioBrick format).

To function as the 'Rock' in the rock-paper-scissors design by Team:Southampton, the part has to be used in conjunction with K180013, and pairs K180014 & K180016, K180017 & K180020.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2760
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 893
    Illegal NheI site found at 916
    Illegal PstI site found at 2760
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 622
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2760
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2760
    Illegal AgeI site found at 3365
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 147