Difference between revisions of "Part:BBa K5115064"
 
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===Introduction===
 
===Introduction===
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This composite part combines all the subunit of the hydrogenase in our ribozyme-assisted polycistronic co-expression system：pRAP. It's worth mention that, in this part, the subunit hoxU is fused with EP, check  [https://parts.igem.org/Part:BBa_K5115059 BBa_K5115059(hoxU fused with EP)]for more details. To learn more about the hydrogenase, please check [https://parts.igem.org/Part:BBa_K5115020 BBa_K5115020(hox and hyp operon)]. To get more information about pRAP, please check [https://2022.igem.wiki/fudan/parts part wiki of 2022 Fudan iGEM ].
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Compared with [https://parts.igem.org/Part:BBa_K5115063 hox and hyp, with EP targeted hoxF], there is only one difference: one links EP with hoxF and another links EP with hoxU. EP ligation of different subunits leads to different effects of hydrogenase's entry into the carboxysome, which further affects the effect of our overall design. After comparison between experiments of these two designs, we make sure that the hoxF-linking-EP one is more satisfying. So, to learn more about the finally adopted part, please click on [https://parts.igem.org/Part:BBa_K5115063 hox and hyp, with EP targeted hoxF].
  
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===Usage and biology===
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===Sequence and Features===
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This part is crucial in  [https://parts.igem.org/Part:BBa_K5115066 BBa_K5115066(mineral, U module)], click on the link to view the experiment details.
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===Characterization===
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Latest revision as of 12:50, 2 October 2024


hox and hyp, with EP targeted hoxU

contributed by Fudan iGEM 2023

Introduction

Compared with hox and hyp, with EP targeted hoxF, there is only one difference: one links EP with hoxF and another links EP with hoxU. EP ligation of different subunits leads to different effects of hydrogenase's entry into the carboxysome, which further affects the effect of our overall design. After comparison between experiments of these two designs, we make sure that the hoxF-linking-EP one is more satisfying. So, to learn more about the finally adopted part, please click on hox and hyp, with EP targeted hoxF.

Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 654
    Illegal BglII site found at 1472
    Illegal BglII site found at 1765
    Illegal BglII site found at 10143
    Illegal BglII site found at 10221
    Illegal BamHI site found at 2059
    Illegal XhoI site found at 99
    Illegal XhoI site found at 2266
    Illegal XhoI site found at 4505
    Illegal XhoI site found at 6334
    Illegal XhoI site found at 7496
    Illegal XhoI site found at 9335
    Illegal XhoI site found at 10151
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 533
    Illegal NgoMIV site found at 1071
    Illegal NgoMIV site found at 1281
    Illegal NgoMIV site found at 1593
    Illegal NgoMIV site found at 2359
    Illegal NgoMIV site found at 3039
    Illegal NgoMIV site found at 5646
    Illegal AgeI site found at 6821
    Illegal AgeI site found at 10104
    Illegal AgeI site found at 11103
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4159
    Illegal BsaI site found at 9925
    Illegal BsaI site found at 10087
    Illegal BsaI.rc site found at 702
    Illegal BsaI.rc site found at 1206
    Illegal SapI.rc site found at 10036


References