Difference between revisions of "Part:BBa K5269008"

Latest revision as of 08:50, 29 September 2024

Surface displayed protein reverse primer 4

This is a reverse primer used for PCR of the Surface displayed protein-SpyTag complex (BBa_K5269021-BBa_K5269024). This primer is also used for Golden Gate Assembly (GGA) to pBluescript II SK(-) which has Golden Gate Assembly Site (BBa_K5269020). This primer is used for GGA of three Surface displayed protein-SpyTag complexes, and is closest to the tac promoter.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K5269008 short</partinfo>
-This is a reverse primer used for PCR of the Surface displayed protein-SpyTag complex (BBa_K5269021-BBa_K5269024). This primer is also used for Golden Gate Assembly (GGA) to pBluescript II SK(-) which has Golden Gate Assembly Site (BBa_K5269020). This primer is used for GGA of three Surface displayed protein-SpyTag complexes, and is closest to the tac promoter(BBa_K5269033).
+This is a reverse primer used for PCR of the Surface displayed protein-SpyTag complex (BBa_K5269021-BBa_K5269024). This primer is also used for Golden Gate Assembly (GGA) to pBluescript II SK(-) which has Golden Gate Assembly Site (BBa_K5269020). This primer is used for GGA of three Surface displayed protein-SpyTag complexes, and is closest to the tac promoter.
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