Difference between revisions of "Part:BBa K5269004"
 
Line 3: Line 3:
 
<partinfo>BBa_K5269004 short</partinfo>
 
<partinfo>BBa_K5269004 short</partinfo>
  
−
This is a forward primer used for PCR of the Surface displayed protein-SpyTag complex (BBa_K5269021-BBa_K5269024). This primer is also used for Golden Gate Assembly (GGA) to pBluescript II SK(-)  which has Golden Gate Assembly Site (BBa_K5269020). This primer is used for GGA of two or more Surface displayed protein-SpyTag complexes, the second one from the tac promoter(BBa_K5269033).
+
This is a forward primer used for PCR of the Surface displayed protein-SpyTag complex (BBa_K5269021-BBa_K5269024). This primer is also used for Golden Gate Assembly (GGA) to pBluescript II SK(-)  which has Golden Gate Assembly Site (BBa_K5269020). This primer is used for GGA of two or more Surface displayed protein-SpyTag complexes, the second one from the tac promoter.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 08:49, 29 September 2024


Surface displayed protein forward primer 2

This is a forward primer used for PCR of the Surface displayed protein-SpyTag complex (BBa_K5269021-BBa_K5269024). This primer is also used for Golden Gate Assembly (GGA) to pBluescript II SK(-) which has Golden Gate Assembly Site (BBa_K5269020). This primer is used for GGA of two or more Surface displayed protein-SpyTag complexes, the second one from the tac promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 7
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 7
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 7
  • 1000
    COMPATIBLE WITH RFC[1000]