Difference between revisions of "Part:BBa K243011:Design"
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===Design Notes=== | ===Design Notes=== | ||
The cloning steps were planned theoretically before we started the work in the wet lab. | The cloning steps were planned theoretically before we started the work in the wet lab. | ||
| − | We used the [https://parts.igem.org/Part:BBa_K243006 long linker] because its lenght of 36bp is a bit shorter | + | We used the [https://parts.igem.org/Part:BBa_K243006 long linker] because its lenght of 36bp is a bit shorter than the split linker we used. |
We had to find a compromise between connection stability and possible sterical interference between the single parts. | We had to find a compromise between connection stability and possible sterical interference between the single parts. | ||
The parts are fused according to [https://parts.igem.org/Assembly_standard_25 RFC 25]. | The parts are fused according to [https://parts.igem.org/Assembly_standard_25 RFC 25]. | ||
Latest revision as of 21:00, 21 October 2009
Strep-DigA-Long Linker-Fok_i
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 278
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The cloning steps were planned theoretically before we started the work in the wet lab. We used the long linker because its lenght of 36bp is a bit shorter than the split linker we used. We had to find a compromise between connection stability and possible sterical interference between the single parts. The parts are fused according to RFC 25. Commented GenBank file
Source
Combined the parts by serial cloning steps.