Difference between revisions of "Part:BBa K5490028"
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| − | + | CasRx is a highly effective RNA-targeting molecule with nuclease activity specifically against single-stranded RNA, making it a versatile tool for gene silencing and antiviral therapies. One of its major advantages over RNA interference (RNAi) is its lower off-target activity, providing more preecision in targeting specific RNA sequences. As a viral protein, CasRx operates independently of the host's cellular machinery, which reduces variability and enhances efficiency. | |
CasRx is the smallest member of the RNA-targeting CRISPR family, allowing for easier delivery into cells while maintaining high specificity and efficiency in cleaving target RNA. Its minimal off-target effects and high efficiency make it ideal for large-scale transcriptome screening, precise gene silencing, and targeting viral RNA in therapeutic applications. | CasRx is the smallest member of the RNA-targeting CRISPR family, allowing for easier delivery into cells while maintaining high specificity and efficiency in cleaving target RNA. Its minimal off-target effects and high efficiency make it ideal for large-scale transcriptome screening, precise gene silencing, and targeting viral RNA in therapeutic applications. | ||
Latest revision as of 00:26, 29 September 2024
CasRx
It’s an inducible endonuclease protein that can target any RNA sequence complementary to a gRNA.
It contains an HA tag for Western blot and Immunohistochemistry analysis, and a mchery reporter with an NLS for separating activity between CasRx and the target RNA.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 499
Illegal EcoRI site found at 919 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 499
Illegal EcoRI site found at 919
Illegal NotI site found at 2908 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 499
Illegal EcoRI site found at 919
Illegal BamHI site found at 2899
Illegal XhoI site found at 2374 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 499
Illegal EcoRI site found at 919 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 499
Illegal EcoRI site found at 919
Illegal NgoMIV site found at 187 - 1000COMPATIBLE WITH RFC[1000]
CasRx is a highly effective RNA-targeting molecule with nuclease activity specifically against single-stranded RNA, making it a versatile tool for gene silencing and antiviral therapies. One of its major advantages over RNA interference (RNAi) is its lower off-target activity, providing more preecision in targeting specific RNA sequences. As a viral protein, CasRx operates independently of the host's cellular machinery, which reduces variability and enhances efficiency.
CasRx is the smallest member of the RNA-targeting CRISPR family, allowing for easier delivery into cells while maintaining high specificity and efficiency in cleaving target RNA. Its minimal off-target effects and high efficiency make it ideal for large-scale transcriptome screening, precise gene silencing, and targeting viral RNA in therapeutic applications.
A key advantage of CasRx over other CRISPR systems is its simplicity--it does not require a PAM sequence or a tracrRNA, making it one of the most straightforward CRISPR systems to implement. CasRx can target virtually any RNA sequence when paired with a guide RNA (gRNA) that is complementary to the target.
Konermann S, Lotfy P, Brideau NJ, Oki J, Shokhirev MN, Hsu PD. Transcriptome Engineering with RNA-Targeting Type VI-D CRISPR Effectors. Cell. 2018 Apr 19;173(3):665-676.e14. doi: 10.1016/j.cell.2018.02.033. Epub 2018 Mar 15. PMID: 29551272; PMCID: PMC5910255.
Chuang YF, Wang PY, Kumar S, Lama S, Lin FL, Liu GS. Methods for in vitro CRISPR/CasRx-Mediated RNA Editing. Front Cell Dev Biol. 2021 Jun 11;9:667879. doi: 10.3389/fcell.2021.667879. PMID: 34178991; PMCID: PMC8226256.