Difference between revisions of "Part:BBa K208051"
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This is a GFP composite with a TorA signal peptide, promoter, RBS, and terminator. This is in pSB1AK3. | This is a GFP composite with a TorA signal peptide, promoter, RBS, and terminator. This is in pSB1AK3. | ||
| − | + | NOTE: The scar between the signal peptide and the protein is not presented correctly in the sequence below. The scar is the Silver-fusion scar (ACTAGA), which allows for in-frame fusion. Additionally, the scars before the signal peptide and after the protein are affected by these parts each having the Silver-fusion prefix and suffix. | |
| + | |||
===Usage and Biology=== | ===Usage and Biology=== | ||
| + | This part can be used to demonstrate GFP secretion via the Tat pathway. | ||
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Latest revision as of 01:12, 23 October 2009
Tet Promoter/RBS/TorA/GFP/Terminator
This is a GFP composite with a TorA signal peptide, promoter, RBS, and terminator. This is in pSB1AK3.
NOTE: The scar between the signal peptide and the protein is not presented correctly in the sequence below. The scar is the Silver-fusion scar (ACTAGA), which allows for in-frame fusion. Additionally, the scars before the signal peptide and after the protein are affected by these parts each having the Silver-fusion prefix and suffix.
Usage and Biology
This part can be used to demonstrate GFP secretion via the Tat pathway.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 216
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 735
Illegal XhoI site found at 636 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]