Difference between revisions of "Part:BBa K5097013:Experience"
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Team Oneonta 2024 used this part to purify sBFP and RFP for studies on the effect of pH on the emission of these fluorescent proteins. We expressed composite parts BBa_K5097016 and BBa_K5097017 in E. coli DH5alpha cells, and lysed the cells by treating with lysozyme and sonication. Proteins were purified via Ni-IMAC chromatography and eluted in 50mM sodium phosphate, 300mM sodium chloride, and 500mM imidazole at pH8.0. Proteins were concentrated and exchanged into PBS via centrifugal diafiltration. | Team Oneonta 2024 used this part to purify sBFP and RFP for studies on the effect of pH on the emission of these fluorescent proteins. We expressed composite parts BBa_K5097016 and BBa_K5097017 in E. coli DH5alpha cells, and lysed the cells by treating with lysozyme and sonication. Proteins were purified via Ni-IMAC chromatography and eluted in 50mM sodium phosphate, 300mM sodium chloride, and 500mM imidazole at pH8.0. Proteins were concentrated and exchanged into PBS via centrifugal diafiltration. | ||
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| + | :::::::::Figure 1: Coomasie stained PAGE gel showing purification of BFP-6XHis and RFP-6XHis | ||
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 17:59, 30 September 2024
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how you used this part and how it worked out.
Applications of BBa_K5097013
Team Oneonta 2024 used this part to purify sBFP and RFP for studies on the effect of pH on the emission of these fluorescent proteins. We expressed composite parts BBa_K5097016 and BBa_K5097017 in E. coli DH5alpha cells, and lysed the cells by treating with lysozyme and sonication. Proteins were purified via Ni-IMAC chromatography and eluted in 50mM sodium phosphate, 300mM sodium chloride, and 500mM imidazole at pH8.0. Proteins were concentrated and exchanged into PBS via centrifugal diafiltration.
- Figure 1: Coomasie stained PAGE gel showing purification of BFP-6XHis and RFP-6XHis
User Reviews
UNIQ8426b3e638c9067f-partinfo-00000000-QINU UNIQ8426b3e638c9067f-partinfo-00000001-QINU