Difference between revisions of "Part:BBa K5097017"
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<partinfo>BBa_K5097017 short</partinfo> | <partinfo>BBa_K5097017 short</partinfo> | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
The 2024 Oneonta iGEM team pHish and CHIPs used RFP as a reporter protein to test the function of pH-sensitive regulatory elements that control gene expression. As a companion to these studies, we investigated what effect pH might have on the fluorescence of RFP. To do this, we generated a constitutively expressed RFP (BBa_K5097015) that is modified on the C-terminal to remove the native stop codon, and then with the addition of an AGVG linker and 6x His tag. A double-stop codon was added at the end. | The 2024 Oneonta iGEM team pHish and CHIPs used RFP as a reporter protein to test the function of pH-sensitive regulatory elements that control gene expression. As a companion to these studies, we investigated what effect pH might have on the fluorescence of RFP. To do this, we generated a constitutively expressed RFP (BBa_K5097015) that is modified on the C-terminal to remove the native stop codon, and then with the addition of an AGVG linker and 6x His tag. A double-stop codon was added at the end. | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Latest revision as of 11:54, 30 September 2024
RFP with 6X His
Usage and Biology
The 2024 Oneonta iGEM team pHish and CHIPs used RFP as a reporter protein to test the function of pH-sensitive regulatory elements that control gene expression. As a companion to these studies, we investigated what effect pH might have on the fluorescence of RFP. To do this, we generated a constitutively expressed RFP (BBa_K5097015) that is modified on the C-terminal to remove the native stop codon, and then with the addition of an AGVG linker and 6x His tag. A double-stop codon was added at the end.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 630
Illegal AgeI site found at 742 - 1000COMPATIBLE WITH RFC[1000]